<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with the Workiva Platform-->
<!--Copyright 2024 Workiva-->
<!--r:1164199d-907a-48b8-8234-6c6f2c33cb08,g:2e7c18b9-a625-4f42-802f-45dc008c9804-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/all" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain"/>
  <link:arcroleRef arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member"/>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="simple" xlink:href="kltr-20231231.xsd#CONSOLIDATEDSTATEMENTSOFOPERATIONS"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/CONSOLIDATEDSTATEMENTSOFOPERATIONS" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_ed6efb77-dcdc-40c7-b6cf-77d8a093588d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_StatementTable_ed6efb77-dcdc-40c7-b6cf-77d8a093588d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_75241f76-b598-4845-b07c-c5b3cdf81995" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ed6efb77-dcdc-40c7-b6cf-77d8a093588d" xlink:to="loc_srt_ProductOrServiceAxis_75241f76-b598-4845-b07c-c5b3cdf81995" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_75241f76-b598-4845-b07c-c5b3cdf81995_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_75241f76-b598-4845-b07c-c5b3cdf81995" xlink:to="loc_srt_ProductsAndServicesDomain_75241f76-b598-4845-b07c-c5b3cdf81995_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_2717e39e-63f0-4099-9e3f-d194cd9c6ecc" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_75241f76-b598-4845-b07c-c5b3cdf81995" xlink:to="loc_srt_ProductsAndServicesDomain_2717e39e-63f0-4099-9e3f-d194cd9c6ecc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_SubscriptionMember_3da3f0f5-401f-4ac4-a71c-34f75bb2ad36" xlink:href="kltr-20231231.xsd#kltr_SubscriptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_2717e39e-63f0-4099-9e3f-d194cd9c6ecc" xlink:to="loc_kltr_SubscriptionMember_3da3f0f5-401f-4ac4-a71c-34f75bb2ad36" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_ProfessionalServicesMember_4544d62e-14f1-4cc6-981c-a0cbc4177b15" xlink:href="kltr-20231231.xsd#kltr_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_2717e39e-63f0-4099-9e3f-d194cd9c6ecc" xlink:to="loc_kltr_ProfessionalServicesMember_4544d62e-14f1-4cc6-981c-a0cbc4177b15" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenuesAbstract_5f1ba49f-1ee4-4e43-8824-f23ee0f20f9e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenuesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_RevenuesAbstract_5f1ba49f-1ee4-4e43-8824-f23ee0f20f9e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_8c0ca636-e63c-405f-867f-3f10dd8a2672" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenuesAbstract_5f1ba49f-1ee4-4e43-8824-f23ee0f20f9e" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_8c0ca636-e63c-405f-867f-3f10dd8a2672" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfRevenueAbstract_36655984-32b2-4538-9caf-8e5c3a05183c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfRevenueAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_CostOfRevenueAbstract_36655984-32b2-4538-9caf-8e5c3a05183c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_f27f4fdf-db5b-47c9-8749-03a0a19ec058" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CostOfRevenueAbstract_36655984-32b2-4538-9caf-8e5c3a05183c" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_f27f4fdf-db5b-47c9-8749-03a0a19ec058" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_88b42653-4be8-4b27-a6bb-ac909a7fe993" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_GrossProfit_88b42653-4be8-4b27-a6bb-ac909a7fe993" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingExpensesAbstract"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_51dcfce2-6235-4308-8ae3-6921176bbb53" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_51dcfce2-6235-4308-8ae3-6921176bbb53" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpense_5f5bc7b7-a680-4efd-b374-9827f119f8eb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SellingAndMarketingExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_SellingAndMarketingExpense_5f5bc7b7-a680-4efd-b374-9827f119f8eb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpense_34af88d9-4e86-44d1-ab49-44c2fb46e279" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GeneralAndAdministrativeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpense_34af88d9-4e86-44d1-ab49-44c2fb46e279" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCosts_02700341-74de-4517-a3dc-e57eb43f36d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_RestructuringCosts_02700341-74de-4517-a3dc-e57eb43f36d4" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherGeneralExpense_c9ea45bb-141d-42ef-a925-9808b7d30753" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherGeneralExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_OtherGeneralExpense_c9ea45bb-141d-42ef-a925-9808b7d30753" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_6c22e024-b30f-4416-ac61-98cb1abbf1ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingExpensesAbstract_af977c93-e313-4a6d-9787-f76fe67fb2ec" xlink:to="loc_us-gaap_OperatingExpenses_6c22e024-b30f-4416-ac61-98cb1abbf1ba" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_f8be4414-2b07-4564-b555-bbb0b4ff48b9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_OperatingIncomeLoss_f8be4414-2b07-4564-b555-bbb0b4ff48b9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_0a8f25de-df3f-495a-abf1-4244c323c0da" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_0a8f25de-df3f-495a-abf1-4244c323c0da" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4541368b-e3bc-4b63-a843-04b57dff9f23" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4541368b-e3bc-4b63-a843-04b57dff9f23" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_689ab255-2e0d-42e0-96ab-6cb3f8f0efc6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_689ab255-2e0d-42e0-96ab-6cb3f8f0efc6" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_e2d11b56-4963-4568-9e53-cf90222b73d2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_NetIncomeLoss_e2d11b56-4963-4568-9e53-cf90222b73d2" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockDividendsAndOtherAdjustments_a46167da-1220-43b6-9a20-48de6bd1f533" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PreferredStockDividendsAndOtherAdjustments"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_PreferredStockDividendsAndOtherAdjustments_a46167da-1220-43b6-9a20-48de6bd1f533" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_d190a63e-85cb-4d3a-8866-cd64aef719fd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_NetIncomeLossAvailableToCommonStockholdersBasic_d190a63e-85cb-4d3a-8866-cd64aef719fd" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EarningsPerShareBasic_dcc6189c-1b3e-478c-99ab-e7c38e91054f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EarningsPerShareBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_EarningsPerShareBasic_dcc6189c-1b3e-478c-99ab-e7c38e91054f" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_80d69605-e0ab-4258-ad91-7fb63d9e07ef" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_WeightedAverageNumberOfSharesOutstandingBasic"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_270ca2c5-509c-4ff9-a8c4-8aac5e2579cd" xlink:to="loc_us-gaap_WeightedAverageNumberOfSharesOutstandingBasic_80d69605-e0ab-4258-ad91-7fb63d9e07ef" xlink:type="arc" order="12"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/CONSOLIDATEDSTATEMENTSOFCONVERTIBLEANDREDEEMABLECONVERTIBLEPREFERREDSTOCKANDSTOCKHOLDERSEQUITYDEFICIT" xlink:type="simple" xlink:href="kltr-20231231.xsd#CONSOLIDATEDSTATEMENTSOFCONVERTIBLEANDREDEEMABLECONVERTIBLEPREFERREDSTOCKANDSTOCKHOLDERSEQUITYDEFICIT"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/CONSOLIDATEDSTATEMENTSOFCONVERTIBLEANDREDEEMABLECONVERTIBLEPREFERREDSTOCKANDSTOCKHOLDERSEQUITYDEFICIT" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_25032375-4fb4-4c9c-823c-a8345e5db517" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_ec8e6be9-067b-4191-b6ce-0270a9d030cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_25032375-4fb4-4c9c-823c-a8345e5db517" xlink:to="loc_us-gaap_StatementTable_ec8e6be9-067b-4191-b6ce-0270a9d030cf" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ec8e6be9-067b-4191-b6ce-0270a9d030cf" xlink:to="loc_us-gaap_StatementClassOfStockAxis_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c" xlink:to="loc_us-gaap_ClassOfStockDomain_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_2620a0e8-013b-4360-b56e-4b4eb2771232" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_c63d26dc-1adb-427e-91b5-c7a9c8ebd49c" xlink:to="loc_us-gaap_ClassOfStockDomain_2620a0e8-013b-4360-b56e-4b4eb2771232" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConvertiblePreferredStockMember_d1af8e9e-f18e-4780-9bf5-6e0c7fbdafb0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2620a0e8-013b-4360-b56e-4b4eb2771232" xlink:to="loc_us-gaap_ConvertiblePreferredStockMember_d1af8e9e-f18e-4780-9bf5-6e0c7fbdafb0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RedeemableConvertiblePreferredStockMember_31a365ce-c44f-4986-88ba-35dc01f15998" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RedeemableConvertiblePreferredStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockDomain_2620a0e8-013b-4360-b56e-4b4eb2771232" xlink:to="loc_us-gaap_RedeemableConvertiblePreferredStockMember_31a365ce-c44f-4986-88ba-35dc01f15998" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_7647e010-7fc8-466e-9c40-71841b825927" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_ec8e6be9-067b-4191-b6ce-0270a9d030cf" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_7647e010-7fc8-466e-9c40-71841b825927" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_7647e010-7fc8-466e-9c40-71841b825927_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7647e010-7fc8-466e-9c40-71841b825927" xlink:to="loc_us-gaap_EquityComponentDomain_7647e010-7fc8-466e-9c40-71841b825927_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_7647e010-7fc8-466e-9c40-71841b825927" xlink:to="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_3d3da187-b8c1-42bb-b9e1-4f58369d94f5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_CommonStockMember_3d3da187-b8c1-42bb-b9e1-4f58369d94f5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivablesFromStockholderMember_ab70ce73-c85c-4b30-acf6-3e905531ece3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivablesFromStockholderMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_ReceivablesFromStockholderMember_ab70ce73-c85c-4b30-acf6-3e905531ece3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapitalMember_d445f2e1-a5fd-4592-8af4-22fc72a62364" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdditionalPaidInCapitalMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_AdditionalPaidInCapitalMember_d445f2e1-a5fd-4592-8af4-22fc72a62364" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsMember_fc1399da-f796-47ed-9c50-9aa22e16b425" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RetainedEarningsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_RetainedEarningsMember_fc1399da-f796-47ed-9c50-9aa22e16b425" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeMember_533c3db3-ae92-47df-91ed-17347fef16d1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ComprehensiveIncomeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_ComprehensiveIncomeMember_533c3db3-ae92-47df-91ed-17347fef16d1" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonMember_1b0ae295-db87-4d82-9ffc-e1d53e7cf850" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockCommonMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_687a06b7-f3de-4007-bd4a-5b0a206b227d" xlink:to="loc_us-gaap_TreasuryStockCommonMember_1b0ae295-db87-4d82-9ffc-e1d53e7cf850" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInTemporaryEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_25032375-4fb4-4c9c-823c-a8345e5db517" xlink:to="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_7c31d39f-384e-4f6a-8748-dd2e9cc77db3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_us-gaap_TemporaryEquitySharesOutstanding_7c31d39f-384e-4f6a-8748-dd2e9cc77db3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_d66a7dbb-2300-4745-865c-9de43fd31793" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_d66a7dbb-2300-4745-865c-9de43fd31793" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityAccretionToRedemptionValue_e06990f9-b3ed-42f5-8c5f-d8dc63607216" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquityAccretionToRedemptionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_us-gaap_TemporaryEquityAccretionToRedemptionValue_e06990f9-b3ed-42f5-8c5f-d8dc63607216" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_TemporaryEquityStockIssuedDuringPeriodSharesNewIssues_7b8bf532-a60c-4a53-a976-232852cb05cc" xlink:href="kltr-20231231.xsd#kltr_TemporaryEquityStockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_kltr_TemporaryEquityStockIssuedDuringPeriodSharesNewIssues_7b8bf532-a60c-4a53-a976-232852cb05cc" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityStockIssuedDuringPeriodValueNewIssues_82816d1e-eda4-44eb-aa36-80f86dda7b53" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquityStockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_us-gaap_TemporaryEquityStockIssuedDuringPeriodValueNewIssues_82816d1e-eda4-44eb-aa36-80f86dda7b53" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockRedemptionDiscount_6dff2865-23be-4c7f-ab48-4fd8ba670b3f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PreferredStockRedemptionDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInTemporaryEquityRollForward_ae3c30f3-d2df-4653-af26-43a85c768e86" xlink:to="loc_us-gaap_PreferredStockRedemptionDiscount_6dff2865-23be-4c7f-ab48-4fd8ba670b3f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquitySharesOutstanding_399b58cd-f36b-49c4-9741-2d2ee4386f3c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquitySharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TemporaryEquityCarryingAmountAttributableToParent_5ab75f81-1951-4b83-9076-f081f7dfb86c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TemporaryEquityCarryingAmountAttributableToParent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncreaseDecreaseInStockholdersEquityRollForward"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_25032375-4fb4-4c9c-823c-a8345e5db517" xlink:to="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_04af026a-7451-4835-8b2e-21f20d9c56f8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_CommonStockSharesOutstanding_04af026a-7451-4835-8b2e-21f20d9c56f8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_1532c7ba-f1cf-4f34-a834-f698501028e6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockholdersEquity_1532c7ba-f1cf-4f34-a834-f698501028e6" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_LoanForgiveness_720b5581-e81c-4798-ab04-5e0ad77f179d" xlink:href="kltr-20231231.xsd#kltr_LoanForgiveness"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_kltr_LoanForgiveness_720b5581-e81c-4798-ab04-5e0ad77f179d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_fe20320b-49e3-4503-9dd1-bbb6febb7935" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_AdjustmentsToAdditionalPaidInCapitalSharebasedCompensationRequisiteServicePeriodRecognitionValue_fe20320b-49e3-4503-9dd1-bbb6febb7935" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_1ef4e5c4-a0e4-4cb0-b1e1-c850b1b07ea9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueStockOptionsExercised_1ef4e5c4-a0e4-4cb0-b1e1-c850b1b07ea9" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_89a0a708-ac19-4f1b-82e4-6f042a3d6af2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesStockOptionsExercised_89a0a708-ac19-4f1b-82e4-6f042a3d6af2" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_90de3f43-0aee-48eb-9487-96386dc5bf3b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueAcquisitions_90de3f43-0aee-48eb-9487-96386dc5bf3b" xlink:type="arc" order="7"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_6f9a5c21-a67e-4dee-8ced-e6c5767cf09c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesAcquisitions"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesAcquisitions_6f9a5c21-a67e-4dee-8ced-e6c5767cf09c" xlink:type="arc" order="8"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PreferredStockAccretionOfRedemptionDiscount_7a55b65c-b1f1-4337-9ea7-d7c655f7ce70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PreferredStockAccretionOfRedemptionDiscount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_PreferredStockAccretionOfRedemptionDiscount_7a55b65c-b1f1-4337-9ea7-d7c655f7ce70" xlink:type="arc" order="9"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_8645a37f-e8f5-4115-8ddb-f9b46a0a243a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesConversionOfConvertibleSecurities_8645a37f-e8f5-4115-8ddb-f9b46a0a243a" xlink:type="arc" order="10"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_e90c4a21-64b5-412e-b8fe-c483c30f6dbf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueConversionOfConvertibleSecurities_e90c4a21-64b5-412e-b8fe-c483c30f6dbf" xlink:type="arc" order="11"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_3d44712f-1047-4b4d-ba25-88a0db5d27c4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodValueNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodValueNewIssues_3d44712f-1047-4b4d-ba25-88a0db5d27c4" xlink:type="arc" order="12"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_939ac19a-46a3-49fa-9d3c-0102673651a4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockIssuedDuringPeriodSharesNewIssues"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_StockIssuedDuringPeriodSharesNewIssues_939ac19a-46a3-49fa-9d3c-0102673651a4" xlink:type="arc" order="13"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StockIssuedDuringPeriodValueConversionOfWarrantsToCommonStock_14d1c81d-6a39-4100-82a1-a20ac15fee52" xlink:href="kltr-20231231.xsd#kltr_StockIssuedDuringPeriodValueConversionOfWarrantsToCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_kltr_StockIssuedDuringPeriodValueConversionOfWarrantsToCommonStock_14d1c81d-6a39-4100-82a1-a20ac15fee52" xlink:type="arc" order="14"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock_780c562d-1a1d-4abb-a1e6-e6fa7c43f8c0" xlink:href="kltr-20231231.xsd#kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock_780c562d-1a1d-4abb-a1e6-e6fa7c43f8c0" xlink:type="arc" order="15"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_6a330280-06cc-45b1-9eda-a8f5fe9ca920" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_6a330280-06cc-45b1-9eda-a8f5fe9ca920" xlink:type="arc" order="16"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_e2ab0a21-c9c7-4cf1-bc0e-38db08db3043" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncreaseDecreaseInStockholdersEquityRollForward_5205d668-a5a3-4d28-95dc-0d40ee686126" xlink:to="loc_us-gaap_NetIncomeLoss_e2ab0a21-c9c7-4cf1-bc0e-38db08db3043" xlink:type="arc" order="17"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockSharesOutstanding_1c4e2e7f-412c-48c5-aa13-08f22fef6883" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockSharesOutstanding"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_fa741c8b-a14b-4809-85df-0c53d56204b7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TreasuryStockCommonShares_71c2e6fe-6824-4435-bb43-5fa51d28f8e8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TreasuryStockCommonShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_25032375-4fb4-4c9c-823c-a8345e5db517" xlink:to="loc_us-gaap_TreasuryStockCommonShares_71c2e6fe-6824-4435-bb43-5fa51d28f8e8" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/SIGNIFICANTACCOUNTINGPOLICIESDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#SIGNIFICANTACCOUNTINGPOLICIESDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/SIGNIFICANTACCOUNTINGPOLICIESDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_be98cf99-742f-449b-a098-0a9dd3e39a63" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_35db1922-f325-4c76-bbfa-536d252f1e5d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_be98cf99-742f-449b-a098-0a9dd3e39a63" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_35db1922-f325-4c76-bbfa-536d252f1e5d" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4cd63297-b5e8-4b6e-86c8-422b80d165d8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_35db1922-f325-4c76-bbfa-536d252f1e5d" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4cd63297-b5e8-4b6e-86c8-422b80d165d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_4cd63297-b5e8-4b6e-86c8-422b80d165d8_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4cd63297-b5e8-4b6e-86c8-422b80d165d8" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_4cd63297-b5e8-4b6e-86c8-422b80d165d8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_03211e3e-2ddc-431e-893e-5c0487a26a44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_4cd63297-b5e8-4b6e-86c8-422b80d165d8" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_03211e3e-2ddc-431e-893e-5c0487a26a44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_f9c37273-efc0-47c7-b6bf-13e58696fb70" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_03211e3e-2ddc-431e-893e-5c0487a26a44" xlink:to="loc_us-gaap_ComputerEquipmentMember_f9c37273-efc0-47c7-b6bf-13e58696fb70" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OfficeEquipmentMember_81f9df71-c7ec-4351-a90c-644280db4690" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OfficeEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_03211e3e-2ddc-431e-893e-5c0487a26a44" xlink:to="loc_us-gaap_OfficeEquipmentMember_81f9df71-c7ec-4351-a90c-644280db4690" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_ffcc5628-1f12-4f68-bc6e-32a5a7ed4337" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_03211e3e-2ddc-431e-893e-5c0487a26a44" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_ffcc5628-1f12-4f68-bc6e-32a5a7ed4337" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_58ce86ac-c8ee-442b-b030-b43269c74ee7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_35db1922-f325-4c76-bbfa-536d252f1e5d" xlink:to="loc_srt_RangeAxis_58ce86ac-c8ee-442b-b030-b43269c74ee7" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_58ce86ac-c8ee-442b-b030-b43269c74ee7_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_RangeAxis_58ce86ac-c8ee-442b-b030-b43269c74ee7" xlink:to="loc_srt_RangeMember_58ce86ac-c8ee-442b-b030-b43269c74ee7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_d9676198-1b7a-4ecf-ab2f-2a337ea8c56b" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_RangeAxis_58ce86ac-c8ee-442b-b030-b43269c74ee7" xlink:to="loc_srt_RangeMember_d9676198-1b7a-4ecf-ab2f-2a337ea8c56b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_6b7c27b1-ebfc-45a2-9930-105341be8f68" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d9676198-1b7a-4ecf-ab2f-2a337ea8c56b" xlink:to="loc_srt_MinimumMember_6b7c27b1-ebfc-45a2-9930-105341be8f68" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_864da9cd-ffd8-4725-ae52-530780ba3827" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_RangeMember_d9676198-1b7a-4ecf-ab2f-2a337ea8c56b" xlink:to="loc_srt_MaximumMember_864da9cd-ffd8-4725-ae52-530780ba3827" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_3c11893b-86f3-4e95-b9f2-7825370ea2e2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_be98cf99-742f-449b-a098-0a9dd3e39a63" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLife_3c11893b-86f3-4e95-b9f2-7825370ea2e2" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentUsefulLifeDescriptionOfTermExtensibleEnumeration_09157631-03ac-4f7d-82d1-922230e860b1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentUsefulLifeDescriptionOfTermExtensibleEnumeration"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_be98cf99-742f-449b-a098-0a9dd3e39a63" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentUsefulLifeDescriptionOfTermExtensibleEnumeration_09157631-03ac-4f7d-82d1-922230e860b1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_b928ed06-18fd-4977-95da-34d2d891cea1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_472b9ac2-c2ca-4911-8ace-9174d3e0f5c2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_b928ed06-18fd-4977-95da-34d2d891cea1" xlink:to="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_472b9ac2-c2ca-4911-8ace-9174d3e0f5c2" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_de92c510-4786-413c-9e4d-3a42a12a6df9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_472b9ac2-c2ca-4911-8ace-9174d3e0f5c2" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_de92c510-4786-413c-9e4d-3a42a12a6df9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_de92c510-4786-413c-9e4d-3a42a12a6df9_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_de92c510-4786-413c-9e4d-3a42a12a6df9" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_de92c510-4786-413c-9e4d-3a42a12a6df9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b351d8fb-8eba-40b0-a856-f35f493530c5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_de92c510-4786-413c-9e4d-3a42a12a6df9" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b351d8fb-8eba-40b0-a856-f35f493530c5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_0865c510-dd6b-4b85-bf8f-ad85690e7339" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b351d8fb-8eba-40b0-a856-f35f493530c5" xlink:to="loc_us-gaap_CustomerRelationshipsMember_0865c510-dd6b-4b85-bf8f-ad85690e7339" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_eb57e1bc-77d6-4d6f-9bf2-f716d2ea8aa6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b351d8fb-8eba-40b0-a856-f35f493530c5" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_eb57e1bc-77d6-4d6f-9bf2-f716d2ea8aa6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradeNamesMember_717347db-1b1b-4114-a77b-48a7e36be706" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradeNamesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_b351d8fb-8eba-40b0-a856-f35f493530c5" xlink:to="loc_us-gaap_TradeNamesMember_717347db-1b1b-4114-a77b-48a7e36be706" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeAxis_8286e55e-a50a-4346-b36d-5c8c9ca4f358" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfFiniteLivedIntangibleAssetsTable_472b9ac2-c2ca-4911-8ace-9174d3e0f5c2" xlink:to="loc_srt_RangeAxis_8286e55e-a50a-4346-b36d-5c8c9ca4f358" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_8286e55e-a50a-4346-b36d-5c8c9ca4f358_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_RangeMember_751f28cc-75e6-44ea-b395-fe80fbdcd191" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_RangeMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MinimumMember_523db464-6b41-4c59-bbff-b72843b7b773" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MinimumMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MaximumMember_ea42480d-d5e3-4f0c-b91f-e8cd46912826" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MaximumMember"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_01acacb5-dbf3-4bd1-a7e8-a191e3af1e13" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsLineItems_b928ed06-18fd-4977-95da-34d2d891cea1" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetUsefulLife_01acacb5-dbf3-4bd1-a7e8-a191e3af1e13" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_fdd6a655-aba6-48fc-8fb5-e1a9e66632b3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EntityWideRevenueMajorCustomerLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_95ef8481-5d87-44a5-b2ae-49ad8d19c921" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_fdd6a655-aba6-48fc-8fb5-e1a9e66632b3" xlink:to="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_95ef8481-5d87-44a5-b2ae-49ad8d19c921" xlink:type="arc" order="3" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_MajorCustomersAxis_be87a62f-3cb3-458d-992c-c34da4c0ec6b" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_MajorCustomersAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_95ef8481-5d87-44a5-b2ae-49ad8d19c921" xlink:to="loc_srt_MajorCustomersAxis_be87a62f-3cb3-458d-992c-c34da4c0ec6b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_be87a62f-3cb3-458d-992c-c34da4c0ec6b_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_MajorCustomersAxis_be87a62f-3cb3-458d-992c-c34da4c0ec6b" xlink:to="loc_srt_NameOfMajorCustomerDomain_be87a62f-3cb3-458d-992c-c34da4c0ec6b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_NameOfMajorCustomerDomain_8c5924e9-210b-4d14-9431-70c9274101d8" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_NameOfMajorCustomerDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_MajorCustomersAxis_be87a62f-3cb3-458d-992c-c34da4c0ec6b" xlink:to="loc_srt_NameOfMajorCustomerDomain_8c5924e9-210b-4d14-9431-70c9274101d8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_MediaAndTelecomMember_6079bd0f-c90e-4631-87e0-8734d44ee0ef" xlink:href="kltr-20231231.xsd#kltr_MediaAndTelecomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_NameOfMajorCustomerDomain_8c5924e9-210b-4d14-9431-70c9274101d8" xlink:to="loc_kltr_MediaAndTelecomMember_6079bd0f-c90e-4631-87e0-8734d44ee0ef" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_95ef8481-5d87-44a5-b2ae-49ad8d19c921" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_7a5652c5-2b6e-4b5a-934e-397bcc0cfb51" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_9be2ba6a-a09c-4c7b-b910-f2e11b64ef93" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_7a5652c5-2b6e-4b5a-934e-397bcc0cfb51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesMember_4847e7d6-011c-44a0-b130-6d001760d08b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_7a5652c5-2b6e-4b5a-934e-397bcc0cfb51" xlink:to="loc_us-gaap_SalesMember_4847e7d6-011c-44a0-b130-6d001760d08b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_e8c588f5-16b5-4d94-b16f-7ca3699c62bb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfEntityWideRevenueByMajorCustomersByReportingSegmentsTable_95ef8481-5d87-44a5-b2ae-49ad8d19c921" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_e8c588f5-16b5-4d94-b16f-7ca3699c62bb" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_e8c588f5-16b5-4d94-b16f-7ca3699c62bb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_e8c588f5-16b5-4d94-b16f-7ca3699c62bb" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_e8c588f5-16b5-4d94-b16f-7ca3699c62bb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_a3461127-bf7f-4e51-81e8-564fa9fc3387" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_e8c588f5-16b5-4d94-b16f-7ca3699c62bb" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_a3461127-bf7f-4e51-81e8-564fa9fc3387" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerConcentrationRiskMember_25eb0c16-9cca-4ecd-afa7-dd4b09d1813c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_a3461127-bf7f-4e51-81e8-564fa9fc3387" xlink:to="loc_us-gaap_CustomerConcentrationRiskMember_25eb0c16-9cca-4ecd-afa7-dd4b09d1813c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_c39369f1-a08e-4a51-b2ba-5c64a4e61b22" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EntityWideRevenueMajorCustomerLineItems_fdd6a655-aba6-48fc-8fb5-e1a9e66632b3" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_c39369f1-a08e-4a51-b2ba-5c64a4e61b22" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/REVENUESFROMCONTRACTSWITHCUSTOMERSDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#REVENUESFROMCONTRACTSWITHCUSTOMERSDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/REVENUESFROMCONTRACTSWITHCUSTOMERSDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueLineItems_c8b1bc2a-c5a6-4ee8-a198-48b14b6f5fd8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DisaggregationOfRevenueTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_c8b1bc2a-c5a6-4ee8-a198-48b14b6f5fd8" xlink:to="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductOrServiceAxis_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductOrServiceAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:to="loc_srt_ProductOrServiceAxis_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_ProductOrServiceAxis_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4" xlink:to="loc_srt_ProductsAndServicesDomain_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_ProductsAndServicesDomain_cb0bf637-e163-438e-96f4-929801501eed" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_ProductsAndServicesDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_ProductOrServiceAxis_f8edd21f-9f2e-4f65-a81c-84d5babd4ea4" xlink:to="loc_srt_ProductsAndServicesDomain_cb0bf637-e163-438e-96f4-929801501eed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_SubscriptionMember_5eb883ae-40d0-487a-ab77-539b639639ea" xlink:href="kltr-20231231.xsd#kltr_SubscriptionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cb0bf637-e163-438e-96f4-929801501eed" xlink:to="loc_kltr_SubscriptionMember_5eb883ae-40d0-487a-ab77-539b639639ea" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_ProfessionalServicesMember_852ddc64-de0e-4949-a5d8-64584e5d5182" xlink:href="kltr-20231231.xsd#kltr_ProfessionalServicesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_ProductsAndServicesDomain_cb0bf637-e163-438e-96f4-929801501eed" xlink:to="loc_kltr_ProfessionalServicesMember_852ddc64-de0e-4949-a5d8-64584e5d5182" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2c071722-cec2-43ca-a5ae-26c154a1b414" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByBenchmarkAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:to="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2c071722-cec2-43ca-a5ae-26c154a1b414" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_2c071722-cec2-43ca-a5ae-26c154a1b414_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2c071722-cec2-43ca-a5ae-26c154a1b414" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_2c071722-cec2-43ca-a5ae-26c154a1b414_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d768a0f9-94d3-40a2-a63e-c4987a158f93" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskBenchmarkDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByBenchmarkAxis_2c071722-cec2-43ca-a5ae-26c154a1b414" xlink:to="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d768a0f9-94d3-40a2-a63e-c4987a158f93" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesRevenueNetMember_7f45e3ad-5928-422f-9850-dd4c99b07c2b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesRevenueNetMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d768a0f9-94d3-40a2-a63e-c4987a158f93" xlink:to="loc_us-gaap_SalesRevenueNetMember_7f45e3ad-5928-422f-9850-dd4c99b07c2b" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SalesMember_0ab7ca96-d150-473c-ac08-435f3b9b4485" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskBenchmarkDomain_d768a0f9-94d3-40a2-a63e-c4987a158f93" xlink:to="loc_us-gaap_SalesMember_0ab7ca96-d150-473c-ac08-435f3b9b4485" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskByTypeAxis_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:to="loc_us-gaap_ConcentrationRiskByTypeAxis_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskTypeDomain_f486874b-a70d-48d2-8fa6-1bb9091a837f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_ConcentrationRiskByTypeAxis_dfd6ca8e-8dbf-4fe5-86d7-1961b970633f" xlink:to="loc_us-gaap_ConcentrationRiskTypeDomain_f486874b-a70d-48d2-8fa6-1bb9091a837f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromRightsConcentrationRiskMember_0bbd8b97-6c55-465b-9461-0360a31897f0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromRightsConcentrationRiskMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ConcentrationRiskTypeDomain_f486874b-a70d-48d2-8fa6-1bb9091a837f" xlink:to="loc_us-gaap_RevenueFromRightsConcentrationRiskMember_0bbd8b97-6c55-465b-9461-0360a31897f0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_2baae7ff-f830-48cb-aeec-9a8389ee727b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DisaggregationOfRevenueTable_69d6b9a2-8ab5-4920-b7a8-7d82bc642b35" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_2baae7ff-f830-48cb-aeec-9a8389ee727b" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_2baae7ff-f830-48cb-aeec-9a8389ee727b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_2baae7ff-f830-48cb-aeec-9a8389ee727b" xlink:to="loc_us-gaap_SegmentDomain_2baae7ff-f830-48cb-aeec-9a8389ee727b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_a1fa4ca4-eba3-4224-9197-11d30442c9c9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_2baae7ff-f830-48cb-aeec-9a8389ee727b" xlink:to="loc_us-gaap_SegmentDomain_a1fa4ca4-eba3-4224-9197-11d30442c9c9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseEducationAndTechnologyMember_d210d220-9bf3-4574-bdbe-ed96f34e1bcc" xlink:href="kltr-20231231.xsd#kltr_EnterpriseEducationAndTechnologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_a1fa4ca4-eba3-4224-9197-11d30442c9c9" xlink:to="loc_kltr_EnterpriseEducationAndTechnologyMember_d210d220-9bf3-4574-bdbe-ed96f34e1bcc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_MediaAndTelecomMember_ba43f250-e902-4b2b-a893-1e44d06f9a51" xlink:href="kltr-20231231.xsd#kltr_MediaAndTelecomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_a1fa4ca4-eba3-4224-9197-11d30442c9c9" xlink:to="loc_kltr_MediaAndTelecomMember_ba43f250-e902-4b2b-a893-1e44d06f9a51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_f51dace0-6c0d-4544-af21-e016c87f7b80" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_c8b1bc2a-c5a6-4ee8-a198-48b14b6f5fd8" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_f51dace0-6c0d-4544-af21-e016c87f7b80" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ConcentrationRiskPercentage1_3cb48774-5f1f-4d5f-89d4-ff183aa33635" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ConcentrationRiskPercentage1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DisaggregationOfRevenueLineItems_c8b1bc2a-c5a6-4ee8-a198-48b14b6f5fd8" xlink:to="loc_us-gaap_ConcentrationRiskPercentage1_3cb48774-5f1f-4d5f-89d4-ff183aa33635" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_681ee301-faa0-4a7f-b786-324d95097d75" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_8ba85e39-3c58-4c77-82f4-441ca3bdaa44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_681ee301-faa0-4a7f-b786-324d95097d75" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_8ba85e39-3c58-4c77-82f4-441ca3bdaa44" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByBillingStatusTypeAxis_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccountsNotesLoansAndFinancingReceivablesByBillingStatusTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionTable_8ba85e39-3c58-4c77-82f4-441ca3bdaa44" xlink:to="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByBillingStatusTypeAxis_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivablesBillingStatusDomain_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivablesBillingStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByBillingStatusTypeAxis_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87" xlink:to="loc_us-gaap_ReceivablesBillingStatusDomain_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReceivablesBillingStatusDomain_019144d3-af99-4879-9e13-cd3b9081c4ed" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReceivablesBillingStatusDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AccountsNotesLoansAndFinancingReceivablesByBillingStatusTypeAxis_3f6e5c20-1e6f-4f07-95bb-78c5dba03f87" xlink:to="loc_us-gaap_ReceivablesBillingStatusDomain_019144d3-af99-4879-9e13-cd3b9081c4ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_BilledRevenuesMember_c80e0c46-27d3-4906-b9e4-add00c54756a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_BilledRevenuesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReceivablesBillingStatusDomain_019144d3-af99-4879-9e13-cd3b9081c4ed" xlink:to="loc_us-gaap_BilledRevenuesMember_c80e0c46-27d3-4906-b9e4-add00c54756a" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_UnbilledRevenuesMember_0a79d4dc-a957-4ef8-9cb8-90c050f2c0f4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_UnbilledRevenuesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ReceivablesBillingStatusDomain_019144d3-af99-4879-9e13-cd3b9081c4ed" xlink:to="loc_us-gaap_UnbilledRevenuesMember_0a79d4dc-a957-4ef8-9cb8-90c050f2c0f4" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligation_cfb89315-3074-4f92-9a5a-aaf222809895" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueRemainingPerformanceObligation"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_681ee301-faa0-4a7f-b786-324d95097d75" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligation_cfb89315-3074-4f92-9a5a-aaf222809895" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_f3e4557e-2ac5-4aeb-a568-76f1d3606e3f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueRemainingPerformanceObligationPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RevenueRemainingPerformanceObligationExpectedTimingOfSatisfactionLineItems_681ee301-faa0-4a7f-b786-324d95097d75" xlink:to="loc_us-gaap_RevenueRemainingPerformanceObligationPercentage_f3e4557e-2ac5-4aeb-a568-76f1d3606e3f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostTable_65210468-3104-4b80-9837-47d9fadbf8bd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_us-gaap_CapitalizedContractCostTable_65210468-3104-4b80-9837-47d9fadbf8bd" xlink:type="arc" order="3" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostAxis_ebadc794-55c8-48d9-a6c6-6920fee94425" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_CapitalizedContractCostTable_65210468-3104-4b80-9837-47d9fadbf8bd" xlink:to="loc_us-gaap_CapitalizedContractCostAxis_ebadc794-55c8-48d9-a6c6-6920fee94425" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostDomain_ebadc794-55c8-48d9-a6c6-6920fee94425_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CapitalizedContractCostAxis_ebadc794-55c8-48d9-a6c6-6920fee94425" xlink:to="loc_us-gaap_CapitalizedContractCostDomain_ebadc794-55c8-48d9-a6c6-6920fee94425_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostDomain_b5daecfb-b5f0-478c-9be0-c1eed56cb9c7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CapitalizedContractCostAxis_ebadc794-55c8-48d9-a6c6-6920fee94425" xlink:to="loc_us-gaap_CapitalizedContractCostDomain_b5daecfb-b5f0-478c-9be0-c1eed56cb9c7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_CostsToObtainContractMember_abb3ee30-f2b6-413a-a942-db2b3810aa95" xlink:href="kltr-20231231.xsd#kltr_CostsToObtainContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostDomain_b5daecfb-b5f0-478c-9be0-c1eed56cb9c7" xlink:to="loc_kltr_CostsToObtainContractMember_abb3ee30-f2b6-413a-a942-db2b3810aa95" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_CostsToFulfillContractMember_53c0ec0d-8629-4624-8fac-e96c429a3903" xlink:href="kltr-20231231.xsd#kltr_CostsToFulfillContractMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostDomain_b5daecfb-b5f0-478c-9be0-c1eed56cb9c7" xlink:to="loc_kltr_CostsToFulfillContractMember_53c0ec0d-8629-4624-8fac-e96c429a3903" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNet_3a84101a-b215-40a6-b1c4-29fb5fd127ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_us-gaap_CapitalizedContractCostNet_3a84101a-b215-40a6-b1c4-29fb5fd127ab" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AdditionsToDeferredContractAcquisitionCostsDuringPeriod_1d7eacd3-6be6-4dff-88f6-15c31fad9e12" xlink:href="kltr-20231231.xsd#kltr_AdditionsToDeferredContractAcquisitionCostsDuringPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_kltr_AdditionsToDeferredContractAcquisitionCostsDuringPeriod_1d7eacd3-6be6-4dff-88f6-15c31fad9e12" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostAmortization_f3d0d25f-e802-4411-ba7c-47165b3881c5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_us-gaap_CapitalizedContractCostAmortization_f3d0d25f-e802-4411-ba7c-47165b3881c5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetCurrent_09bf68ed-a4d0-4484-836e-bebab42bbe3f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostNetCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_us-gaap_CapitalizedContractCostNetCurrent_09bf68ed-a4d0-4484-836e-bebab42bbe3f" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNetNoncurrent_b3741e00-a3e0-4921-be6d-06f93eadd371" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostNetNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CapitalizedContractCostLineItems_9166fb6e-7a2d-47d9-a783-ff99573e126a" xlink:to="loc_us-gaap_CapitalizedContractCostNetNoncurrent_b3741e00-a3e0-4921-be6d-06f93eadd371" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNet_e98fea13-253b-4a21-ab78-7d915d9e54b5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CapitalizedContractCostNet_c1395662-96b6-4e2d-a82c-f9b13feabe4b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CapitalizedContractCostNet"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/InvestmentsDebtandEquitySecuritiesDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#InvestmentsDebtandEquitySecuritiesDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/InvestmentsDebtandEquitySecuritiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_98c9f008-a385-4b10-9c9b-43a7868348ba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtSecuritiesAvailableForSaleTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_98c9f008-a385-4b10-9c9b-43a7868348ba" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DebtSecuritiesAvailableForSaleTable_98c9f008-a385-4b10-9c9b-43a7868348ba" xlink:to="loc_us-gaap_FinancialInstrumentAxis_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_c6c3f96e-a8a1-4ce8-ba5b-cebe5e6a9a54" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateBondSecuritiesMember_47024b60-d8b3-4d12-b32b-f3348c2d88f9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateBondSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:to="loc_us-gaap_CorporateBondSecuritiesMember_47024b60-d8b3-4d12-b32b-f3348c2d88f9" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MunicipalBondsMember_618e880b-4b31-41fd-84a2-e8ee9a3ac174" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MunicipalBondsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:to="loc_us-gaap_MunicipalBondsMember_618e880b-4b31-41fd-84a2-e8ee9a3ac174" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_67f777a3-ae44-4b54-be97-8667db1a3788" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_67f777a3-ae44-4b54-be97-8667db1a3788" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_9cf43429-4366-421c-baa9-c4d56d7785d7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:to="loc_us-gaap_CommercialPaperMember_9cf43429-4366-421c-baa9-c4d56d7785d7" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AgencySecuritiesMember_a44308df-ecfb-456d-a1fd-593593475650" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AgencySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_55d44fe6-c1cb-4df8-954e-d5b6fa5e976a" xlink:to="loc_us-gaap_AgencySecuritiesMember_a44308df-ecfb-456d-a1fd-593593475650" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost_8e813d62-b7b1-4d99-b122-11a337b710d3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearAmortizedCost_8e813d62-b7b1-4d99-b122-11a337b710d3" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AvailableForSaleMaturitiesWithinOneYearGrossUnrealizedGains_d717e8a0-dec3-4808-b779-1a04d43703ed" xlink:href="kltr-20231231.xsd#kltr_AvailableForSaleMaturitiesWithinOneYearGrossUnrealizedGains"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_kltr_AvailableForSaleMaturitiesWithinOneYearGrossUnrealizedGains_d717e8a0-dec3-4808-b779-1a04d43703ed" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AvailableForSaleMaturesWithinOneYearGrossUnrealizedLosses_3db0c68e-5f79-46ec-bc33-a128a8d57318" xlink:href="kltr-20231231.xsd#kltr_AvailableForSaleMaturesWithinOneYearGrossUnrealizedLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_kltr_AvailableForSaleMaturesWithinOneYearGrossUnrealizedLosses_3db0c68e-5f79-46ec-bc33-a128a8d57318" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue_ba0deb6d-42ef-411d-9520-d8c0e6a8a332" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesWithinOneYearFairValue_ba0deb6d-42ef-411d-9520-d8c0e6a8a332" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_a6fab99d-9675-41cd-80e8-8724405a3797" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsFairValue_a6fab99d-9675-41cd-80e8-8724405a3797" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedLosses_6e21e599-78ea-4735-95b8-cb4421859317" xlink:href="kltr-20231231.xsd#kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedLosses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedLosses_6e21e599-78ea-4735-95b8-cb4421859317" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedGains_a156e022-47b4-4f18-ac7e-4dccba41968c" xlink:href="kltr-20231231.xsd#kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedGains"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_kltr_AvailableForSaleMaturesAfterOneYearGrossUnrealizedGains_a156e022-47b4-4f18-ac7e-4dccba41968c" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost_171e45f1-8185-480a-b0fb-b8cde71f2ee6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ScheduleOfAvailableForSaleSecuritiesLineItems_3c394128-729d-41bc-acbf-6600f4837d60" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtMaturitiesAfterOneThroughFiveYearsAmortizedCost_171e45f1-8185-480a-b0fb-b8cde71f2ee6" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/FAIRVALUEMEASUREMENTSDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#FAIRVALUEMEASUREMENTSDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/FAIRVALUEMEASUREMENTSDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAxis_6bf4837d-4b2a-41eb-8781-8ceda1af740e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:to="loc_us-gaap_CashAndCashEquivalentsAxis_6bf4837d-4b2a-41eb-8781-8ceda1af740e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_6bf4837d-4b2a-41eb-8781-8ceda1af740e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_6bf4837d-4b2a-41eb-8781-8ceda1af740e" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_6bf4837d-4b2a-41eb-8781-8ceda1af740e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_368155b3-6ccc-4c38-a021-a4e4b18821a7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CashAndCashEquivalentsAxis_6bf4837d-4b2a-41eb-8781-8ceda1af740e" xlink:to="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_368155b3-6ccc-4c38-a021-a4e4b18821a7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MoneyMarketFundsMember_0af8b4a3-fcff-466a-8270-637c8aa9d5d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MoneyMarketFundsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_RestrictedCashAndCashEquivalentsCashAndCashEquivalentsMember_368155b3-6ccc-4c38-a021-a4e4b18821a7" xlink:to="loc_us-gaap_MoneyMarketFundsMember_0af8b4a3-fcff-466a-8270-637c8aa9d5d4" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_e9065355-1c47-4bd4-811b-7fa57d612949" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByFairValueHierarchyLevelAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:to="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_e9065355-1c47-4bd4-811b-7fa57d612949" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_e9065355-1c47-4bd4-811b-7fa57d612949_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_e9065355-1c47-4bd4-811b-7fa57d612949" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_e9065355-1c47-4bd4-811b-7fa57d612949_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_828cff23-4616-4ce9-af3c-d085df2e5ae8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsFairValueHierarchyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByFairValueHierarchyLevelAxis_e9065355-1c47-4bd4-811b-7fa57d612949" xlink:to="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_828cff23-4616-4ce9-af3c-d085df2e5ae8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel1Member_760629c2-7e8c-4181-bf70-8d0e71abffd5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel1Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_828cff23-4616-4ce9-af3c-d085df2e5ae8" xlink:to="loc_us-gaap_FairValueInputsLevel1Member_760629c2-7e8c-4181-bf70-8d0e71abffd5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueInputsLevel2Member_c78a5c94-3281-4ff7-bd6d-9bcd4639a994" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueInputsLevel2Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementsFairValueHierarchyDomain_828cff23-4616-4ce9-af3c-d085df2e5ae8" xlink:to="loc_us-gaap_FairValueInputsLevel2Member_c78a5c94-3281-4ff7-bd6d-9bcd4639a994" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueByMeasurementFrequencyAxis_0414d1cf-112d-4b19-89e7-50aea2cb91be" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueByMeasurementFrequencyAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:to="loc_us-gaap_FairValueByMeasurementFrequencyAxis_0414d1cf-112d-4b19-89e7-50aea2cb91be" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_0414d1cf-112d-4b19-89e7-50aea2cb91be_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_0414d1cf-112d-4b19-89e7-50aea2cb91be" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_0414d1cf-112d-4b19-89e7-50aea2cb91be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d8b3798-cea2-4711-9587-bd44c468be9c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementFrequencyDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FairValueByMeasurementFrequencyAxis_0414d1cf-112d-4b19-89e7-50aea2cb91be" xlink:to="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d8b3798-cea2-4711-9587-bd44c468be9c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FairValueMeasurementsRecurringMember_877eb1e3-9a81-4484-8932-089e530b90c6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FairValueMeasurementsRecurringMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueMeasurementFrequencyDomain_9d8b3798-cea2-4711-9587-bd44c468be9c" xlink:to="loc_us-gaap_FairValueMeasurementsRecurringMember_877eb1e3-9a81-4484-8932-089e530b90c6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FinancialInstrumentAxis_065b3815-0707-474c-9794-64d054334032" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FinancialInstrumentAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:to="loc_us-gaap_FinancialInstrumentAxis_065b3815-0707-474c-9794-64d054334032" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_065b3815-0707-474c-9794-64d054334032_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FinancialInstrumentAxis_065b3815-0707-474c-9794-64d054334032" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_065b3815-0707-474c-9794-64d054334032_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FinancialInstrumentAxis_065b3815-0707-474c-9794-64d054334032" xlink:to="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CorporateBondSecuritiesMember_8c240d5e-255b-4d60-9f59-8cfe84a7e7f8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CorporateBondSecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_CorporateBondSecuritiesMember_8c240d5e-255b-4d60-9f59-8cfe84a7e7f8" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_MunicipalBondsMember_a08f4b05-b84a-4570-b23d-e3b69d9eadbc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_MunicipalBondsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_MunicipalBondsMember_a08f4b05-b84a-4570-b23d-e3b69d9eadbc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_USTreasurySecuritiesMember_001e3206-a77e-4188-b10f-cdbb00963628" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_USTreasurySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_USTreasurySecuritiesMember_001e3206-a77e-4188-b10f-cdbb00963628" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommercialPaperMember_c4510b28-022b-4dc2-8400-286df9a320d4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommercialPaperMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_CommercialPaperMember_c4510b28-022b-4dc2-8400-286df9a320d4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AgencySecuritiesMember_42d904ec-f122-4841-b8b0-b7ab24b5c5ef" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AgencySecuritiesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_AgencySecuritiesMember_42d904ec-f122-4841-b8b0-b7ab24b5c5ef" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeMember_69364bd8-37e6-47db-8090-348edd50e02a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_DerivativeMember_69364bd8-37e6-47db-8090-348edd50e02a" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepositsMember_9b4d52e7-7ef8-44e8-ab3f-a5779533d83b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DepositsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_TransfersAndServicingOfFinancialInstrumentsTypesOfFinancialInstrumentsDomain_14e47457-b930-469d-b4fa-5cd4280852f7" xlink:to="loc_us-gaap_DepositsMember_9b4d52e7-7ef8-44e8-ab3f-a5779533d83b" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_58e6eb89-327c-46cc-81ca-21362eea5e92" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisTable_57138670-bc36-42fb-8c0e-05595d95fad7" xlink:to="loc_us-gaap_HedgingDesignationAxis_58e6eb89-327c-46cc-81ca-21362eea5e92" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_58e6eb89-327c-46cc-81ca-21362eea5e92_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_58e6eb89-327c-46cc-81ca-21362eea5e92" xlink:to="loc_us-gaap_HedgingDesignationDomain_58e6eb89-327c-46cc-81ca-21362eea5e92_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_1867133c-51e4-4b3d-b2be-2e183de5181d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_58e6eb89-327c-46cc-81ca-21362eea5e92" xlink:to="loc_us-gaap_HedgingDesignationDomain_1867133c-51e4-4b3d-b2be-2e183de5181d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_057855e7-3ce8-4439-873e-fd8422ff63ff" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_1867133c-51e4-4b3d-b2be-2e183de5181d" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_057855e7-3ce8-4439-873e-fd8422ff63ff" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_85164177-0ee9-42e3-a722-e74f6afcfd58" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CashAndCashEquivalentsFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_CashAndCashEquivalentsFairValueDisclosure_85164177-0ee9-42e3-a722-e74f6afcfd58" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_1c5ad943-c0a7-4913-9a74-08605f6caa1c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesCurrent_1c5ad943-c0a7-4913-9a74-08605f6caa1c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent_e15210d6-a35e-41af-8590-a8c2529902d5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_AvailableForSaleSecuritiesDebtSecuritiesNoncurrent_e15210d6-a35e-41af-8590-a8c2529902d5" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherRestrictedAssetsCurrent_e1ba1920-10f0-472f-9a5a-e65d4f1f1929" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherRestrictedAssetsCurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_OtherRestrictedAssetsCurrent_e1ba1920-10f0-472f-9a5a-e65d4f1f1929" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCurrencyContractAssetFairValueDisclosure_c04efd2f-e558-420f-a595-9f0986750112" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ForeignCurrencyContractAssetFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_ForeignCurrencyContractAssetFairValueDisclosure_c04efd2f-e558-420f-a595-9f0986750112" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherRestrictedAssetsNoncurrent_e833d0f6-cb0d-47ee-ae9f-0ed8dce94ea0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherRestrictedAssetsNoncurrent"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_OtherRestrictedAssetsNoncurrent_e833d0f6-cb0d-47ee-ae9f-0ed8dce94ea0" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ForeignCurrencyContractsLiabilityFairValueDisclosure_643703b8-eff9-443e-ba1d-0e21c910adeb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ForeignCurrencyContractsLiabilityFairValueDisclosure"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FairValueAssetsAndLiabilitiesMeasuredOnRecurringAndNonrecurringBasisLineItems_68018b93-7095-4975-b7b5-edf936ecbb12" xlink:to="loc_us-gaap_ForeignCurrencyContractsLiabilityFairValueDisclosure_643703b8-eff9-443e-ba1d-0e21c910adeb" xlink:type="arc" order="6"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/DerivativeInstrumentsandHedgingActivitiesDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#DerivativeInstrumentsandHedgingActivitiesDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/DerivativeInstrumentsandHedgingActivitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_3a581b01-2740-419e-88c7-4ea46c802c64" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_17b6eb31-77c7-4bb1-ad7b-f9453e16a883" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_3a581b01-2740-419e-88c7-4ea46c802c64" xlink:to="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_17b6eb31-77c7-4bb1-ad7b-f9453e16a883" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_e62b3136-34b9-4356-bc3a-59a326001def" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_17b6eb31-77c7-4bb1-ad7b-f9453e16a883" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_e62b3136-34b9-4356-bc3a-59a326001def" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_e62b3136-34b9-4356-bc3a-59a326001def_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_e62b3136-34b9-4356-bc3a-59a326001def" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_e62b3136-34b9-4356-bc3a-59a326001def_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_e62b3136-34b9-4356-bc3a-59a326001def" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_2216e90a-a6d9-4e33-95bb-b02d8d6cf1f6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:to="loc_us-gaap_CostOfSalesMember_2216e90a-a6d9-4e33-95bb-b02d8d6cf1f6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_538dd9ce-eaef-4108-88c9-62278cb60dc1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_538dd9ce-eaef-4108-88c9-62278cb60dc1" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_ce8aef83-11eb-45b0-a13b-3e609e0ebc6b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_ce8aef83-11eb-45b0-a13b-3e609e0ebc6b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_03db7058-949e-4e9f-8633-27e0e6499596" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_03db7058-949e-4e9f-8633-27e0e6499596" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringChargesMember_9e3f5d0e-ce84-460b-adab-4e7da6906733" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringChargesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_d4da0ac2-e3a6-4146-bd2a-ed0d5a94848a" xlink:to="loc_us-gaap_RestructuringChargesMember_9e3f5d0e-ce84-460b-adab-4e7da6906733" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationAxis_eb953149-8b71-46f8-8458-f16f57bcf767" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresTable_17b6eb31-77c7-4bb1-ad7b-f9453e16a883" xlink:to="loc_us-gaap_HedgingDesignationAxis_eb953149-8b71-46f8-8458-f16f57bcf767" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_eb953149-8b71-46f8-8458-f16f57bcf767_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_HedgingDesignationAxis_eb953149-8b71-46f8-8458-f16f57bcf767" xlink:to="loc_us-gaap_HedgingDesignationDomain_eb953149-8b71-46f8-8458-f16f57bcf767_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_HedgingDesignationDomain_8ce98269-c681-48d2-9711-a1e7b99d4ac5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_HedgingDesignationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_HedgingDesignationAxis_eb953149-8b71-46f8-8458-f16f57bcf767" xlink:to="loc_us-gaap_HedgingDesignationDomain_8ce98269-c681-48d2-9711-a1e7b99d4ac5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DesignatedAsHedgingInstrumentMember_849a2953-f90f-4eee-811f-2b1d981e93fb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DesignatedAsHedgingInstrumentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_HedgingDesignationDomain_8ce98269-c681-48d2-9711-a1e7b99d4ac5" xlink:to="loc_us-gaap_DesignatedAsHedgingInstrumentMember_849a2953-f90f-4eee-811f-2b1d981e93fb" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeGainLossOnDerivativeNet_e8d4ebf8-6138-4f35-a2c4-c6bba036da1d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeGainLossOnDerivativeNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_3a581b01-2740-419e-88c7-4ea46c802c64" xlink:to="loc_us-gaap_DerivativeGainLossOnDerivativeNet_e8d4ebf8-6138-4f35-a2c4-c6bba036da1d" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DerivativeGainLossStatementOfIncomeOrComprehensiveIncomeExtensibleEnumeration_1f4e551b-162a-4003-a5f8-3b2ebd707f03" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DerivativeGainLossStatementOfIncomeOrComprehensiveIncomeExtensibleEnumeration"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_DerivativeInstrumentsAndHedgingActivitiesDisclosuresLineItems_3a581b01-2740-419e-88c7-4ea46c802c64" xlink:to="loc_us-gaap_DerivativeGainLossStatementOfIncomeOrComprehensiveIncomeExtensibleEnumeration_1f4e551b-162a-4003-a5f8-3b2ebd707f03" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/CONSOLIDATEDBALANCESHEETCOMPONENTSDetails2PPE" xlink:type="simple" xlink:href="kltr-20231231.xsd#CONSOLIDATEDBALANCESHEETCOMPONENTSDetails2PPE"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/CONSOLIDATEDBALANCESHEETCOMPONENTSDetails2PPE" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentLineItems_c39210a2-f86e-4493-8708-e9b483a89c8c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f8318e48-6766-49a7-bf57-3fa43c09a7a2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfPropertyPlantAndEquipmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_c39210a2-f86e-4493-8708-e9b483a89c8c" xlink:to="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f8318e48-6766-49a7-bf57-3fa43c09a7a2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_243005c6-450a-4e36-92cc-833ecdcece3a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentByTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfPropertyPlantAndEquipmentTable_f8318e48-6766-49a7-bf57-3fa43c09a7a2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_243005c6-450a-4e36-92cc-833ecdcece3a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_243005c6-450a-4e36-92cc-833ecdcece3a_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_243005c6-450a-4e36-92cc-833ecdcece3a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_243005c6-450a-4e36-92cc-833ecdcece3a_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentByTypeAxis_243005c6-450a-4e36-92cc-833ecdcece3a" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComputerEquipmentMember_e13899d8-402c-43ac-a006-2d5f9d69cecf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ComputerEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:to="loc_us-gaap_ComputerEquipmentMember_e13899d8-402c-43ac-a006-2d5f9d69cecf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OfficeEquipmentMember_8680f317-0cba-4f3f-8ff8-c987d3a82159" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OfficeEquipmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:to="loc_us-gaap_OfficeEquipmentMember_8680f317-0cba-4f3f-8ff8-c987d3a82159" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdImprovementsMember_5af3db61-1829-4701-b303-ed227a96ab4a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:to="loc_us-gaap_LeaseholdImprovementsMember_5af3db61-1829-4701-b303-ed227a96ab4a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SoftwareDevelopmentMember_b9219460-d53b-43dc-99c8-83582b4a6736" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SoftwareDevelopmentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:to="loc_us-gaap_SoftwareDevelopmentMember_b9219460-d53b-43dc-99c8-83582b4a6736" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LeaseholdsAndLeaseholdImprovementsMember_3ba875a8-b22a-4c5f-af89-31dadda0bf32" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LeaseholdsAndLeaseholdImprovementsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentTypeDomain_d0bb50c3-0808-4792-b710-2dc9139c459a" xlink:to="loc_us-gaap_LeaseholdsAndLeaseholdImprovementsMember_3ba875a8-b22a-4c5f-af89-31dadda0bf32" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_9c344eec-2858-420b-9f1f-17ae4ae270ce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_c39210a2-f86e-4493-8708-e9b483a89c8c" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_9c344eec-2858-420b-9f1f-17ae4ae270ce" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_0b4ed95b-6f8c-445f-abe7-d3b5c9dba001" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_c39210a2-f86e-4493-8708-e9b483a89c8c" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_0b4ed95b-6f8c-445f-abe7-d3b5c9dba001" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_454cf3b6-bf40-4289-a608-b2ec028b5733" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentLineItems_c39210a2-f86e-4493-8708-e9b483a89c8c" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_454cf3b6-bf40-4289-a608-b2ec028b5733" xlink:type="arc" order="2"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/GOODWILLANDINTANGIBLEASSETSNETDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#GOODWILLANDINTANGIBLEASSETSNETDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/GOODWILLANDINTANGIBLEASSETSNETDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_446aabe9-54f5-459d-a888-fdc4e7e3a692" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:to="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_446aabe9-54f5-459d-a888-fdc4e7e3a692" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9cad2326-06ce-42c1-877c-2d7779aae9ce" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAcquiredFiniteLivedIntangibleAssetByMajorClassTable_446aabe9-54f5-459d-a888-fdc4e7e3a692" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9cad2326-06ce-42c1-877c-2d7779aae9ce" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9cad2326-06ce-42c1-877c-2d7779aae9ce_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9cad2326-06ce-42c1-877c-2d7779aae9ce" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_9cad2326-06ce-42c1-877c-2d7779aae9ce_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5678d9f3-56a8-4232-88d2-9c6e424675a2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsByMajorClassAxis_9cad2326-06ce-42c1-877c-2d7779aae9ce" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5678d9f3-56a8-4232-88d2-9c6e424675a2" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_05f8da2d-9b4b-4549-a61f-1d0ec74b5ffc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TechnologyBasedIntangibleAssetsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5678d9f3-56a8-4232-88d2-9c6e424675a2" xlink:to="loc_us-gaap_TechnologyBasedIntangibleAssetsMember_05f8da2d-9b4b-4549-a61f-1d0ec74b5ffc" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CustomerRelationshipsMember_44b88782-c4fd-4367-b987-548a18ca8443" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CustomerRelationshipsMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5678d9f3-56a8-4232-88d2-9c6e424675a2" xlink:to="loc_us-gaap_CustomerRelationshipsMember_44b88782-c4fd-4367-b987-548a18ca8443" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TradeNamesMember_c5e4eeaa-ed2d-4e41-8185-c6747086cb42" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TradeNamesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsMajorClassNameDomain_5678d9f3-56a8-4232-88d2-9c6e424675a2" xlink:to="loc_us-gaap_TradeNamesMember_c5e4eeaa-ed2d-4e41-8185-c6747086cb42" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_0622c36e-0b23-46b7-8887-c047d292f98c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:to="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsWeightedAverageUsefulLife_0622c36e-0b23-46b7-8887-c047d292f98c" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_38a4f86a-251f-45a4-ac58-9a1a1f3e4e2f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_38a4f86a-251f-45a4-ac58-9a1a1f3e4e2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_0249ea45-1245-483d-be07-647b618d18ec" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_0249ea45-1245-483d-be07-647b618d18ec" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_370aa70c-fc8c-492d-bd3a-c6c245905f76" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AcquiredFiniteLivedIntangibleAssetsLineItems_e245acc5-bd44-4fcf-8ad3-1e355049188c" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_370aa70c-fc8c-492d-bd3a-c6c245905f76" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GoodwillLineItems_920d73b7-99fe-4e2b-9413-3492bd0131ab" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GoodwillLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfGoodwillTable_e5622514-f6f7-463a-9f18-016d865cc42b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfGoodwillTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_GoodwillLineItems_920d73b7-99fe-4e2b-9413-3492bd0131ab" xlink:to="loc_us-gaap_ScheduleOfGoodwillTable_e5622514-f6f7-463a-9f18-016d865cc42b" xlink:type="arc" order="2" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_3b34be2e-ec51-4346-9121-1e29e1617721" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfGoodwillTable_e5622514-f6f7-463a-9f18-016d865cc42b" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_3b34be2e-ec51-4346-9121-1e29e1617721" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_3b34be2e-ec51-4346-9121-1e29e1617721_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3b34be2e-ec51-4346-9121-1e29e1617721" xlink:to="loc_us-gaap_SegmentDomain_3b34be2e-ec51-4346-9121-1e29e1617721_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_2e2d5e5d-cc2a-4ce6-a325-53adff207f09" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_3b34be2e-ec51-4346-9121-1e29e1617721" xlink:to="loc_us-gaap_SegmentDomain_2e2d5e5d-cc2a-4ce6-a325-53adff207f09" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_MediaAndTelecomMember_3e33fb47-cd7f-4ea1-9436-5a7be04e58bf" xlink:href="kltr-20231231.xsd#kltr_MediaAndTelecomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_2e2d5e5d-cc2a-4ce6-a325-53adff207f09" xlink:to="loc_kltr_MediaAndTelecomMember_3e33fb47-cd7f-4ea1-9436-5a7be04e58bf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseEducationAndTechnologyMember_de8adcbd-7898-40d5-80d3-539155082bf9" xlink:href="kltr-20231231.xsd#kltr_EnterpriseEducationAndTechnologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_2e2d5e5d-cc2a-4ce6-a325-53adff207f09" xlink:to="loc_kltr_EnterpriseEducationAndTechnologyMember_de8adcbd-7898-40d5-80d3-539155082bf9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Goodwill_110f4824-89a1-456f-9955-3394cd3b801f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_Goodwill"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_GoodwillLineItems_920d73b7-99fe-4e2b-9413-3492bd0131ab" xlink:to="loc_us-gaap_Goodwill_110f4824-89a1-456f-9955-3394cd3b801f" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/INCOMETAXESDetails1" xlink:type="simple" xlink:href="kltr-20231231.xsd#INCOMETAXESDetails1"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/INCOMETAXESDetails1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_479bd317-9ca0-4c10-85ed-776c2c00c633" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_0781fe89-898d-4f69-9145-21fe0469c849" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_479bd317-9ca0-4c10-85ed-776c2c00c633" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_0781fe89-898d-4f69-9145-21fe0469c849" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityAxis_280998d0-9ea2-4258-96b4-c26597b1746c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_0781fe89-898d-4f69-9145-21fe0469c849" xlink:to="loc_us-gaap_IncomeTaxAuthorityAxis_280998d0-9ea2-4258-96b4-c26597b1746c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_280998d0-9ea2-4258-96b4-c26597b1746c_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_280998d0-9ea2-4258-96b4-c26597b1746c" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_280998d0-9ea2-4258-96b4-c26597b1746c_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxAuthorityDomain_c3a452c1-ad14-43f3-9468-addc16013ebd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxAuthorityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeTaxAuthorityAxis_280998d0-9ea2-4258-96b4-c26597b1746c" xlink:to="loc_us-gaap_IncomeTaxAuthorityDomain_c3a452c1-ad14-43f3-9468-addc16013ebd" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DomesticDestinationMember_16462cee-8878-450c-a8d1-79b4653cd0fe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DomesticDestinationMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_c3a452c1-ad14-43f3-9468-addc16013ebd" xlink:to="loc_us-gaap_DomesticDestinationMember_16462cee-8878-450c-a8d1-79b4653cd0fe" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StateAndLocalJurisdictionMember_5f92027a-f94b-4cca-a138-e939d1205f02" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StateAndLocalJurisdictionMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeTaxAuthorityDomain_c3a452c1-ad14-43f3-9468-addc16013ebd" xlink:to="loc_us-gaap_StateAndLocalJurisdictionMember_5f92027a-f94b-4cca-a138-e939d1205f02" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwards_5bd4a60f-1513-47c8-b122-a8b24a30446f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwards"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_479bd317-9ca0-4c10-85ed-776c2c00c633" xlink:to="loc_us-gaap_OperatingLossCarryforwards_5bd4a60f-1513-47c8-b122-a8b24a30446f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxCreditCarryforwardAmount_c0130f54-f5bc-4dba-9efb-cc0daff19965" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_TaxCreditCarryforwardAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_479bd317-9ca0-4c10-85ed-776c2c00c633" xlink:to="loc_us-gaap_TaxCreditCarryforwardAmount_c0130f54-f5bc-4dba-9efb-cc0daff19965" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/INCOMETAXESDetails3" xlink:type="simple" xlink:href="kltr-20231231.xsd#INCOMETAXESDetails3"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/INCOMETAXESDetails3" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsLineItems_98163a47-2ff2-4ff5-96b6-aa1c75582344" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLossCarryforwardsTable_8e4ebc9d-2ecf-4b4a-b421-d3563df9dc3a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingLossCarryforwardsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_98163a47-2ff2-4ff5-96b6-aa1c75582344" xlink:to="loc_us-gaap_OperatingLossCarryforwardsTable_8e4ebc9d-2ecf-4b4a-b421-d3563df9dc3a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseAxis_e52eb70d-4ad8-4555-95ca-5095b768c37e" xlink:href="kltr-20231231.xsd#kltr_EnterpriseAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_OperatingLossCarryforwardsTable_8e4ebc9d-2ecf-4b4a-b421-d3563df9dc3a" xlink:to="loc_kltr_EnterpriseAxis_e52eb70d-4ad8-4555-95ca-5095b768c37e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseDomain_e52eb70d-4ad8-4555-95ca-5095b768c37e_default" xlink:href="kltr-20231231.xsd#kltr_EnterpriseDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_kltr_EnterpriseAxis_e52eb70d-4ad8-4555-95ca-5095b768c37e" xlink:to="loc_kltr_EnterpriseDomain_e52eb70d-4ad8-4555-95ca-5095b768c37e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseDomain_7ef6fe8f-db8d-44b6-98ab-a41ee7870d85" xlink:href="kltr-20231231.xsd#kltr_EnterpriseDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_kltr_EnterpriseAxis_e52eb70d-4ad8-4555-95ca-5095b768c37e" xlink:to="loc_kltr_EnterpriseDomain_7ef6fe8f-db8d-44b6-98ab-a41ee7870d85" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_BeneficiaryEnterpriseMember_286899b1-8db2-47b5-91bb-31b8f3d393e0" xlink:href="kltr-20231231.xsd#kltr_BeneficiaryEnterpriseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_EnterpriseDomain_7ef6fe8f-db8d-44b6-98ab-a41ee7870d85" xlink:to="loc_kltr_BeneficiaryEnterpriseMember_286899b1-8db2-47b5-91bb-31b8f3d393e0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxReconciliationTaxExemptIncome_2b6d0750-ca1e-41e0-910f-5a66d680d030" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxReconciliationTaxExemptIncome"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_OperatingLossCarryforwardsLineItems_98163a47-2ff2-4ff5-96b6-aa1c75582344" xlink:to="loc_us-gaap_IncomeTaxReconciliationTaxExemptIncome_2b6d0750-ca1e-41e0-910f-5a66d680d030" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/NETLOSSPERSHAREATTRIBUTABLETOCOMMONSTOCKHOLDERSDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#NETLOSSPERSHAREATTRIBUTABLETOCOMMONSTOCKHOLDERSDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/NETLOSSPERSHAREATTRIBUTABLETOCOMMONSTOCKHOLDERSDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_0e153d36-87c2-4d8f-a895-60445dfcf92e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_b9de1885-11a1-4cef-9293-886232858af2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_0e153d36-87c2-4d8f-a895-60445dfcf92e" xlink:to="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_b9de1885-11a1-4cef-9293-886232858af2" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfAntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareTable_b9de1885-11a1-4cef-9293-886232858af2" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesNameDomain_ca11ffc4-7742-4a51-81fb-75e14ae76f23" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareByAntidilutiveSecuritiesAxis_e6ed8c1a-e57c-4aa6-8814-b845b9b0ecfb" xlink:to="loc_us-gaap_AntidilutiveSecuritiesNameDomain_ca11ffc4-7742-4a51-81fb-75e14ae76f23" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_ec0d81b0-ac81-401c-a5fd-24563b2ce657" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesNameDomain_ca11ffc4-7742-4a51-81fb-75e14ae76f23" xlink:to="loc_us-gaap_EmployeeStockMember_ec0d81b0-ac81-401c-a5fd-24563b2ce657" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_10084318-1435-4fde-9c90-5b6a49483230" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareLineItems_0e153d36-87c2-4d8f-a895-60445dfcf92e" xlink:to="loc_us-gaap_AntidilutiveSecuritiesExcludedFromComputationOfEarningsPerShareAmount_10084318-1435-4fde-9c90-5b6a49483230" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8730d23e-9f2c-418d-8306-ae7b27c5c753" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8730d23e-9f2c-418d-8306-ae7b27c5c753" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementBusinessSegmentsAxis_081e8418-eded-4c21-8259-51b0b7124956" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementBusinessSegmentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_8730d23e-9f2c-418d-8306-ae7b27c5c753" xlink:to="loc_us-gaap_StatementBusinessSegmentsAxis_081e8418-eded-4c21-8259-51b0b7124956" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_081e8418-eded-4c21-8259-51b0b7124956_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_081e8418-eded-4c21-8259-51b0b7124956" xlink:to="loc_us-gaap_SegmentDomain_081e8418-eded-4c21-8259-51b0b7124956_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentDomain_64171654-5ea7-4c2a-8ac9-c1efd3c64678" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementBusinessSegmentsAxis_081e8418-eded-4c21-8259-51b0b7124956" xlink:to="loc_us-gaap_SegmentDomain_64171654-5ea7-4c2a-8ac9-c1efd3c64678" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_EnterpriseEducationAndTechnologyMember_73abdfa6-341e-4030-bc56-50c6cdc39aae" xlink:href="kltr-20231231.xsd#kltr_EnterpriseEducationAndTechnologyMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64171654-5ea7-4c2a-8ac9-c1efd3c64678" xlink:to="loc_kltr_EnterpriseEducationAndTechnologyMember_73abdfa6-341e-4030-bc56-50c6cdc39aae" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_MediaAndTelecomMember_02470c61-620e-474a-8913-e81d2cad057e" xlink:href="kltr-20231231.xsd#kltr_MediaAndTelecomMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentDomain_64171654-5ea7-4c2a-8ac9-c1efd3c64678" xlink:to="loc_kltr_MediaAndTelecomMember_02470c61-620e-474a-8913-e81d2cad057e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b60a3004-cb05-4c0b-b903-d2dd58438a99" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_b60a3004-cb05-4c0b-b903-d2dd58438a99" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GrossProfit_51c8a778-5da6-4a47-9591-2542ecbdb335" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GrossProfit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_GrossProfit_51c8a778-5da6-4a47-9591-2542ecbdb335" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_923a760c-d043-4190-943d-ca99b8b53b8c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OperatingExpenses"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_OperatingExpenses_923a760c-d043-4190-943d-ca99b8b53b8c" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NonoperatingIncomeExpense_ca9ba431-46d3-4bf3-8d7c-60283e24a6e4" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NonoperatingIncomeExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_NonoperatingIncomeExpense_ca9ba431-46d3-4bf3-8d7c-60283e24a6e4" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_f25d55e9-ff7b-4230-b332-d64b49f4652a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_f25d55e9-ff7b-4230-b332-d64b49f4652a" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_6fa8583a-e2da-4143-a88b-003c01c4a065" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NetIncomeLoss"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_fc9187b9-7cab-41f8-a8ae-2e574a28f494" xlink:to="loc_us-gaap_NetIncomeLoss_6fa8583a-e2da-4143-a88b-003c01c4a065" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails2" xlink:type="simple" xlink:href="kltr-20231231.xsd#REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails2"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails2" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_b2d64ed6-910f-4adc-8706-9bf19a6e2618" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f13fcd65-4205-40df-be37-decfd327228a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_b2d64ed6-910f-4adc-8706-9bf19a6e2618" xlink:to="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f13fcd65-4205-40df-be37-decfd327228a" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_d0f93283-3610-4452-ae19-7dd45a3bbdc9" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfSegmentReportingInformationBySegmentTable_f13fcd65-4205-40df-be37-decfd327228a" xlink:to="loc_srt_StatementGeographicalAxis_d0f93283-3610-4452-ae19-7dd45a3bbdc9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_d0f93283-3610-4452-ae19-7dd45a3bbdc9_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_d0f93283-3610-4452-ae19-7dd45a3bbdc9" xlink:to="loc_srt_SegmentGeographicalDomain_d0f93283-3610-4452-ae19-7dd45a3bbdc9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_948c7d47-cbd3-4069-b608-81303f5a6970" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_d0f93283-3610-4452-ae19-7dd45a3bbdc9" xlink:to="loc_srt_SegmentGeographicalDomain_948c7d47-cbd3-4069-b608-81303f5a6970" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_13a823b3-58aa-47aa-ac17-c28493617af6" xlink:href="https://xbrl.sec.gov/country/2023/country-2023.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_948c7d47-cbd3-4069-b608-81303f5a6970" xlink:to="loc_country_US_13a823b3-58aa-47aa-ac17-c28493617af6" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EMEAMember_9511b779-9b54-4bd9-9559-c7f82901f4d3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EMEAMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_948c7d47-cbd3-4069-b608-81303f5a6970" xlink:to="loc_us-gaap_EMEAMember_9511b779-9b54-4bd9-9559-c7f82901f4d3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_OtherCountriesMember_10efadd0-c510-47b8-8d68-27138fd75a65" xlink:href="kltr-20231231.xsd#kltr_OtherCountriesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_948c7d47-cbd3-4069-b608-81303f5a6970" xlink:to="loc_kltr_OtherCountriesMember_10efadd0-c510-47b8-8d68-27138fd75a65" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_81ab3bb2-ca1a-486b-bbb2-055d70168cb0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_b2d64ed6-910f-4adc-8706-9bf19a6e2618" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_81ab3bb2-ca1a-486b-bbb2-055d70168cb0" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails3" xlink:type="simple" xlink:href="kltr-20231231.xsd#REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails3"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/REPORTABLESEGMENTSANDGEOGRAPHICALINFORMATIONDetails3" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SegmentReportingInformationLineItems_6c7a59c0-450d-47e6-855e-4e30e470451c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SegmentReportingInformationLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_eac95bb3-5aca-4396-bdcf-251e0223a258" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6c7a59c0-450d-47e6-855e-4e30e470451c" xlink:to="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_eac95bb3-5aca-4396-bdcf-251e0223a258" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_StatementGeographicalAxis_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_StatementGeographicalAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfRevenuesFromExternalCustomersAndLongLivedAssetsTable_eac95bb3-5aca-4396-bdcf-251e0223a258" xlink:to="loc_srt_StatementGeographicalAxis_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8_default" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_srt_StatementGeographicalAxis_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8" xlink:to="loc_srt_SegmentGeographicalDomain_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_srt_SegmentGeographicalDomain_cf177885-d513-4117-9ca4-fa1da1c7d5e7" xlink:href="https://xbrl.fasb.org/srt/2023/elts/srt-2023.xsd#srt_SegmentGeographicalDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_srt_StatementGeographicalAxis_c8fc46f0-1ec0-4a4b-9685-0a0986c801e8" xlink:to="loc_srt_SegmentGeographicalDomain_cf177885-d513-4117-9ca4-fa1da1c7d5e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_US_3e0365d9-76f1-4236-880f-3df875a3e00e" xlink:href="https://xbrl.sec.gov/country/2023/country-2023.xsd#country_US"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cf177885-d513-4117-9ca4-fa1da1c7d5e7" xlink:to="loc_country_US_3e0365d9-76f1-4236-880f-3df875a3e00e" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_country_IL_3ee1c7a3-9531-4927-89b4-fce80c6ebd51" xlink:href="https://xbrl.sec.gov/country/2023/country-2023.xsd#country_IL"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cf177885-d513-4117-9ca4-fa1da1c7d5e7" xlink:to="loc_country_IL_3ee1c7a3-9531-4927-89b4-fce80c6ebd51" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_OtherCountriesMember_782679cf-5250-46d0-a8fd-4b3b6c4d13ea" xlink:href="kltr-20231231.xsd#kltr_OtherCountriesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_srt_SegmentGeographicalDomain_cf177885-d513-4117-9ca4-fa1da1c7d5e7" xlink:to="loc_kltr_OtherCountriesMember_782679cf-5250-46d0-a8fd-4b3b6c4d13ea" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NoncurrentAssets_984b967f-acb2-4ca9-a464-068200284aac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_NoncurrentAssets"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_SegmentReportingInformationLineItems_6c7a59c0-450d-47e6-855e-4e30e470451c" xlink:to="loc_us-gaap_NoncurrentAssets_984b967f-acb2-4ca9-a464-068200284aac" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/LONGTERMLOANDetails" xlink:type="simple" xlink:href="kltr-20231231.xsd#LONGTERMLOANDetails"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/LONGTERMLOANDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_9e2b77ea-0b7a-4451-aa46-7031987b3728" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:to="loc_us-gaap_CreditFacilityAxis_9e2b77ea-0b7a-4451-aa46-7031987b3728" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_9e2b77ea-0b7a-4451-aa46-7031987b3728_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_9e2b77ea-0b7a-4451-aa46-7031987b3728" xlink:to="loc_us-gaap_CreditFacilityDomain_9e2b77ea-0b7a-4451-aa46-7031987b3728_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_72ca6272-32c2-43a8-bca2-d599a38049dc" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_9e2b77ea-0b7a-4451-aa46-7031987b3728" xlink:to="loc_us-gaap_CreditFacilityDomain_72ca6272-32c2-43a8-bca2-d599a38049dc" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_TermLoanFacilityMember_3e51a2bb-5da7-4a41-892a-b87b709477e7" xlink:href="kltr-20231231.xsd#kltr_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_72ca6272-32c2-43a8-bca2-d599a38049dc" xlink:to="loc_kltr_TermLoanFacilityMember_3e51a2bb-5da7-4a41-892a-b87b709477e7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevolvingCreditFacilityMember_22222cb5-bbee-48d5-a005-5f007cd41006" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RevolvingCreditFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_72ca6272-32c2-43a8-bca2-d599a38049dc" xlink:to="loc_us-gaap_RevolvingCreditFacilityMember_22222cb5-bbee-48d5-a005-5f007cd41006" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_01219572-3fed-4eb9-ad82-243e82842638" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:to="loc_us-gaap_VariableRateAxis_01219572-3fed-4eb9-ad82-243e82842638" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_01219572-3fed-4eb9-ad82-243e82842638_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_01219572-3fed-4eb9-ad82-243e82842638" xlink:to="loc_us-gaap_VariableRateDomain_01219572-3fed-4eb9-ad82-243e82842638_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_01219572-3fed-4eb9-ad82-243e82842638" xlink:to="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EurodollarMember_33a92346-f955-4549-952c-0f74a59d7271" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EurodollarMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:to="loc_us-gaap_EurodollarMember_33a92346-f955-4549-952c-0f74a59d7271" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AlternateBaseRateMember_b454e362-6ae3-446c-8055-adef9599ce7e" xlink:href="kltr-20231231.xsd#kltr_AlternateBaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:to="loc_kltr_AlternateBaseRateMember_b454e362-6ae3-446c-8055-adef9599ce7e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_e7e4da50-df25-4754-9f06-1cf5099aa01e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:to="loc_us-gaap_SecuredOvernightFinancingRateSofrOvernightIndexSwapRateMember_e7e4da50-df25-4754-9f06-1cf5099aa01e" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_FederalFundsEffectiveRateMember_c8223617-0dce-46e1-a1da-8bdad74c0a7e" xlink:href="kltr-20231231.xsd#kltr_FederalFundsEffectiveRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6a5cb377-d258-47b2-b8e1-99882150ca03" xlink:to="loc_kltr_FederalFundsEffectiveRateMember_c8223617-0dce-46e1-a1da-8bdad74c0a7e" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StatedPercentageTypeAxis_4b314964-1381-4433-a6ef-62c8a7e494d2" xlink:href="kltr-20231231.xsd#kltr_StatedPercentageTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:to="loc_kltr_StatedPercentageTypeAxis_4b314964-1381-4433-a6ef-62c8a7e494d2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StatedPercentageTypeDomain_4b314964-1381-4433-a6ef-62c8a7e494d2_default" xlink:href="kltr-20231231.xsd#kltr_StatedPercentageTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_kltr_StatedPercentageTypeAxis_4b314964-1381-4433-a6ef-62c8a7e494d2" xlink:to="loc_kltr_StatedPercentageTypeDomain_4b314964-1381-4433-a6ef-62c8a7e494d2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:href="kltr-20231231.xsd#kltr_StatedPercentageTypeDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_kltr_StatedPercentageTypeAxis_4b314964-1381-4433-a6ef-62c8a7e494d2" xlink:to="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_InterestMarginMember_829bac6d-5a7a-49be-ab56-88cefdff7b68" xlink:href="kltr-20231231.xsd#kltr_InterestMarginMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:to="loc_kltr_InterestMarginMember_829bac6d-5a7a-49be-ab56-88cefdff7b68" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_CreditAdjustmentSpreadMember_49cb9ffa-aea0-4fed-b002-38a26c8ae988" xlink:href="kltr-20231231.xsd#kltr_CreditAdjustmentSpreadMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:to="loc_kltr_CreditAdjustmentSpreadMember_49cb9ffa-aea0-4fed-b002-38a26c8ae988" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestRateFloorMember_20ed932e-0f1c-46ac-9cbc-a64e66816f8a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_InterestRateFloorMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:to="loc_us-gaap_InterestRateFloorMember_20ed932e-0f1c-46ac-9cbc-a64e66816f8a" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_FederalFundsEffectiveRateMember_0aa74272-9134-4803-8c8f-cb0ea29f791c" xlink:href="kltr-20231231.xsd#kltr_FederalFundsEffectiveRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_StatedPercentageTypeDomain_d196c07a-f823-4934-a96d-4ebc85d1a6ae" xlink:to="loc_kltr_FederalFundsEffectiveRateMember_0aa74272-9134-4803-8c8f-cb0ea29f791c" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_InstallmentPaymentDateAxis_0b7835ef-5d3a-445e-8ebd-1b04bde853f9" xlink:href="kltr-20231231.xsd#kltr_InstallmentPaymentDateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_e724f48a-31f7-48f5-bfee-9240338e55fc" xlink:to="loc_kltr_InstallmentPaymentDateAxis_0b7835ef-5d3a-445e-8ebd-1b04bde853f9" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_InstallmentPaymentDateDomain_0b7835ef-5d3a-445e-8ebd-1b04bde853f9_default" xlink:href="kltr-20231231.xsd#kltr_InstallmentPaymentDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_kltr_InstallmentPaymentDateAxis_0b7835ef-5d3a-445e-8ebd-1b04bde853f9" xlink:to="loc_kltr_InstallmentPaymentDateDomain_0b7835ef-5d3a-445e-8ebd-1b04bde853f9_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_InstallmentPaymentDateDomain_de43b70d-5fc7-4a8d-95b4-6ca9d8d645b7" xlink:href="kltr-20231231.xsd#kltr_InstallmentPaymentDateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_kltr_InstallmentPaymentDateAxis_0b7835ef-5d3a-445e-8ebd-1b04bde853f9" xlink:to="loc_kltr_InstallmentPaymentDateDomain_de43b70d-5fc7-4a8d-95b4-6ca9d8d645b7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_OnDecember312023ThroughSeptember302024Member_ae2ff3fa-1f50-4986-b8d1-c056538e2853" xlink:href="kltr-20231231.xsd#kltr_OnDecember312023ThroughSeptember302024Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_InstallmentPaymentDateDomain_de43b70d-5fc7-4a8d-95b4-6ca9d8d645b7" xlink:to="loc_kltr_OnDecember312023ThroughSeptember302024Member_ae2ff3fa-1f50-4986-b8d1-c056538e2853" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_OnDecember312024ThroughSeptember302025Member_c854a1c0-cb32-4dcc-ba42-700381289277" xlink:href="kltr-20231231.xsd#kltr_OnDecember312024ThroughSeptember302025Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_InstallmentPaymentDateDomain_de43b70d-5fc7-4a8d-95b4-6ca9d8d645b7" xlink:to="loc_kltr_OnDecember312024ThroughSeptember302025Member_c854a1c0-cb32-4dcc-ba42-700381289277" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_OnAndAfterDecember312025Member_6f741e21-6ed3-448f-99df-da604d92a9c1" xlink:href="kltr-20231231.xsd#kltr_OnAndAfterDecember312025Member"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_kltr_InstallmentPaymentDateDomain_de43b70d-5fc7-4a8d-95b4-6ca9d8d645b7" xlink:to="loc_kltr_OnAndAfterDecember312025Member_6f741e21-6ed3-448f-99df-da604d92a9c1" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_c36b64ca-a83d-4494-877c-762b39e6193f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_c36b64ca-a83d-4494-877c-762b39e6193f" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_15913c48-6168-4202-b4b6-c60b29b921f9" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_LineOfCreditFacilityMaximumBorrowingCapacity_15913c48-6168-4202-b4b6-c60b29b921f9" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_9060c8de-ec88-41e5-bd72-eb860b9c362d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_9060c8de-ec88-41e5-bd72-eb860b9c362d" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_3b7c91f7-e3cc-493a-970e-b0ba1506db5d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentInterestRateStatedPercentage"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_DebtInstrumentInterestRateStatedPercentage_3b7c91f7-e3cc-493a-970e-b0ba1506db5d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_69fe1dc3-357e-4126-be2e-6875d9307f96" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_69fe1dc3-357e-4126-be2e-6875d9307f96" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentPeriodicPayment_4f892bf5-47cb-4cf1-852c-36d06c1387e1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentPeriodicPayment"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_76468e94-fce5-4814-a04c-c1153f87ceba" xlink:to="loc_us-gaap_DebtInstrumentPeriodicPayment_4f892bf5-47cb-4cf1-852c-36d06c1387e1" xlink:type="arc" order="5"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/LONGTERMLOANDetails2" xlink:type="simple" xlink:href="kltr-20231231.xsd#LONGTERMLOANDetails2"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/LONGTERMLOANDetails2" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_f148b468-fc64-436b-8747-edaf5c93d84c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_b7d33cff-4be1-40e2-a160-b67d3202d2eb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_f148b468-fc64-436b-8747-edaf5c93d84c" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_b7d33cff-4be1-40e2-a160-b67d3202d2eb" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_ff8fd879-b699-4eeb-bada-6890fb4211e7" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_b7d33cff-4be1-40e2-a160-b67d3202d2eb" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_ff8fd879-b699-4eeb-bada-6890fb4211e7" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_ff8fd879-b699-4eeb-bada-6890fb4211e7_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_ff8fd879-b699-4eeb-bada-6890fb4211e7" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_ff8fd879-b699-4eeb-bada-6890fb4211e7_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_a42dc633-ad67-44ca-b552-4b9244819f44" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_ff8fd879-b699-4eeb-bada-6890fb4211e7" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_a42dc633-ad67-44ca-b552-4b9244819f44" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_0bf5443c-aed6-44f8-a9f9-cd0237c6e154" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_b7d33cff-4be1-40e2-a160-b67d3202d2eb" xlink:to="loc_us-gaap_CreditFacilityAxis_0bf5443c-aed6-44f8-a9f9-cd0237c6e154" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_0bf5443c-aed6-44f8-a9f9-cd0237c6e154_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_0bf5443c-aed6-44f8-a9f9-cd0237c6e154" xlink:to="loc_us-gaap_CreditFacilityDomain_0bf5443c-aed6-44f8-a9f9-cd0237c6e154_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_b4dc1c2c-bda3-4181-846b-298323832eac" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_0bf5443c-aed6-44f8-a9f9-cd0237c6e154" xlink:to="loc_us-gaap_CreditFacilityDomain_b4dc1c2c-bda3-4181-846b-298323832eac" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateAxis_4b4a8550-e28a-48b4-b876-1a0f9b201e6d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_b7d33cff-4be1-40e2-a160-b67d3202d2eb" xlink:to="loc_us-gaap_VariableRateAxis_4b4a8550-e28a-48b4-b876-1a0f9b201e6d" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_4b4a8550-e28a-48b4-b876-1a0f9b201e6d_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_VariableRateAxis_4b4a8550-e28a-48b4-b876-1a0f9b201e6d" xlink:to="loc_us-gaap_VariableRateDomain_4b4a8550-e28a-48b4-b876-1a0f9b201e6d_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_VariableRateDomain_6375f2a4-8853-4ac5-9c97-1bf53a83cdd3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_VariableRateDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_VariableRateAxis_4b4a8550-e28a-48b4-b876-1a0f9b201e6d" xlink:to="loc_us-gaap_VariableRateDomain_6375f2a4-8853-4ac5-9c97-1bf53a83cdd3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EurodollarMember_f3cc2d81-4251-41bd-ba34-cb7173662092" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EurodollarMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6375f2a4-8853-4ac5-9c97-1bf53a83cdd3" xlink:to="loc_us-gaap_EurodollarMember_f3cc2d81-4251-41bd-ba34-cb7173662092" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_AlternateBaseRateMember_85339a88-4de7-46b7-a01b-c79c1be8904d" xlink:href="kltr-20231231.xsd#kltr_AlternateBaseRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6375f2a4-8853-4ac5-9c97-1bf53a83cdd3" xlink:to="loc_kltr_AlternateBaseRateMember_85339a88-4de7-46b7-a01b-c79c1be8904d" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_FederalFundsEffectiveRateMember_5372ab30-f7b3-4ff5-9fec-331e5a24a8fb" xlink:href="kltr-20231231.xsd#kltr_FederalFundsEffectiveRateMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_VariableRateDomain_6375f2a4-8853-4ac5-9c97-1bf53a83cdd3" xlink:to="loc_kltr_FederalFundsEffectiveRateMember_5372ab30-f7b3-4ff5-9fec-331e5a24a8fb" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_5648b514-9388-4d47-9e7f-8d36c16c51c1" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentBasisSpreadOnVariableRate1"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_f148b468-fc64-436b-8747-edaf5c93d84c" xlink:to="loc_us-gaap_DebtInstrumentBasisSpreadOnVariableRate1_5648b514-9388-4d47-9e7f-8d36c16c51c1" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_11934ce2-cf4d-47ea-ba14-58b07f38ff16" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_f148b468-fc64-436b-8747-edaf5c93d84c" xlink:to="loc_us-gaap_LineOfCreditFacilityInterestRateAtPeriodEnd_11934ce2-cf4d-47ea-ba14-58b07f38ff16" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/LONGTERMLOANDetails4" xlink:type="simple" xlink:href="kltr-20231231.xsd#LONGTERMLOANDetails4"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/LONGTERMLOANDetails4" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityTable_146c9516-1688-4c5b-9e76-194096372a81" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:to="loc_us-gaap_LineOfCreditFacilityTable_146c9516-1688-4c5b-9e76-194096372a81" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityAxis_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_146c9516-1688-4c5b-9e76-194096372a81" xlink:to="loc_us-gaap_LineOfCreditFacilityAxis_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LineOfCreditFacilityLenderDomain_20d86c0f-afb4-4a70-ae49-567282885f20" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LineOfCreditFacilityLenderDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_LineOfCreditFacilityAxis_5fde8b48-2a10-4ee1-b17d-cab6ae66cecb" xlink:to="loc_us-gaap_LineOfCreditFacilityLenderDomain_20d86c0f-afb4-4a70-ae49-567282885f20" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityAxis_b986ab2d-808d-4b62-9f2f-a82fe025ec1b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_LineOfCreditFacilityTable_146c9516-1688-4c5b-9e76-194096372a81" xlink:to="loc_us-gaap_CreditFacilityAxis_b986ab2d-808d-4b62-9f2f-a82fe025ec1b" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_b986ab2d-808d-4b62-9f2f-a82fe025ec1b_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_CreditFacilityAxis_b986ab2d-808d-4b62-9f2f-a82fe025ec1b" xlink:to="loc_us-gaap_CreditFacilityDomain_b986ab2d-808d-4b62-9f2f-a82fe025ec1b_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CreditFacilityDomain_a0dfae9e-5292-49d3-95ac-9b6e25786449" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CreditFacilityDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_CreditFacilityAxis_b986ab2d-808d-4b62-9f2f-a82fe025ec1b" xlink:to="loc_us-gaap_CreditFacilityDomain_a0dfae9e-5292-49d3-95ac-9b6e25786449" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_TermLoanFacilityMember_19248ebd-fed8-43a5-a455-dbb8b8278493" xlink:href="kltr-20231231.xsd#kltr_TermLoanFacilityMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_CreditFacilityDomain_a0dfae9e-5292-49d3-95ac-9b6e25786449" xlink:to="loc_kltr_TermLoanFacilityMember_19248ebd-fed8-43a5-a455-dbb8b8278493" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_a2567968-7778-47df-9152-746b159a4161" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_a2567968-7778-47df-9152-746b159a4161" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_3ff9f47e-7c1a-47e4-8d47-6cdb3c87b700" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_3ff9f47e-7c1a-47e4-8d47-6cdb3c87b700" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_a528710d-14a6-4989-ba0e-b23341f85bcd" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_a528710d-14a6-4989-ba0e-b23341f85bcd" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DebtInstrumentFaceAmount_19706bfe-a9a4-41a1-9e2a-04ace5a1ee83" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_DebtInstrumentFaceAmount"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_LineOfCreditFacilityLineItems_fbd56a13-5a05-4734-931e-00fc9d0e7a97" xlink:to="loc_us-gaap_DebtInstrumentFaceAmount_19706bfe-a9a4-41a1-9e2a-04ace5a1ee83" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails3CommonStockFutureIssuance" xlink:type="simple" xlink:href="kltr-20231231.xsd#STOCKHOLDERSEQUITYDetails3CommonStockFutureIssuance"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails3CommonStockFutureIssuance" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_64767a94-1f4a-4612-9478-ddded354dc7d" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_906a75b9-8ce5-40c7-b218-8bfae20df9a3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_64767a94-1f4a-4612-9478-ddded354dc7d" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_906a75b9-8ce5-40c7-b218-8bfae20df9a3" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_906a75b9-8ce5-40c7-b218-8bfae20df9a3" xlink:to="loc_us-gaap_AwardTypeAxis_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a5938c9f-e192-4874-9260-90d63476120c" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_70994d6c-fe76-4da9-b4c6-0e93aa05d3eb" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a5938c9f-e192-4874-9260-90d63476120c" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeStockMember_be576a55-7df6-469f-a512-7b5f02a32705" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EmployeeStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a5938c9f-e192-4874-9260-90d63476120c" xlink:to="loc_us-gaap_EmployeeStockMember_be576a55-7df6-469f-a512-7b5f02a32705" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockMember_71ece2f8-ed29-4b75-a118-fec16516b038" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_a5938c9f-e192-4874-9260-90d63476120c" xlink:to="loc_us-gaap_RestrictedStockMember_71ece2f8-ed29-4b75-a118-fec16516b038" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_898a1cf0-fd1d-42a9-94b7-9cb72f946212" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_906a75b9-8ce5-40c7-b218-8bfae20df9a3" xlink:to="loc_us-gaap_PlanNameAxis_898a1cf0-fd1d-42a9-94b7-9cb72f946212" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_898a1cf0-fd1d-42a9-94b7-9cb72f946212_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_898a1cf0-fd1d-42a9-94b7-9cb72f946212" xlink:to="loc_us-gaap_PlanNameDomain_898a1cf0-fd1d-42a9-94b7-9cb72f946212_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_5ab133e9-570f-442d-bd6c-18436a2bded3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_898a1cf0-fd1d-42a9-94b7-9cb72f946212" xlink:to="loc_us-gaap_PlanNameDomain_5ab133e9-570f-442d-bd6c-18436a2bded3" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_A2021PlanMember_ef626fe0-58cf-4eb4-947e-3829e35efa96" xlink:href="kltr-20231231.xsd#kltr_A2021PlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_5ab133e9-570f-442d-bd6c-18436a2bded3" xlink:to="loc_kltr_A2021PlanMember_ef626fe0-58cf-4eb4-947e-3829e35efa96" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_0541461b-1e56-4bcd-92c3-0341f9761ba2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_906a75b9-8ce5-40c7-b218-8bfae20df9a3" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_0541461b-1e56-4bcd-92c3-0341f9761ba2" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_0541461b-1e56-4bcd-92c3-0341f9761ba2_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_0541461b-1e56-4bcd-92c3-0341f9761ba2" xlink:to="loc_us-gaap_EquityComponentDomain_0541461b-1e56-4bcd-92c3-0341f9761ba2_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_5871f31a-b4a5-4e38-b7cc-ad9c675e2e2f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_0541461b-1e56-4bcd-92c3-0341f9761ba2" xlink:to="loc_us-gaap_EquityComponentDomain_5871f31a-b4a5-4e38-b7cc-ad9c675e2e2f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockMember_47a640f0-c450-48d9-a56f-9d106a51d9cf" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_5871f31a-b4a5-4e38-b7cc-ad9c675e2e2f" xlink:to="loc_us-gaap_CommonStockMember_47a640f0-c450-48d9-a56f-9d106a51d9cf" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_b670956e-df4a-4733-963f-a696fc6e8714" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CommonStockCapitalSharesReservedForFutureIssuance"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_64767a94-1f4a-4612-9478-ddded354dc7d" xlink:to="loc_us-gaap_CommonStockCapitalSharesReservedForFutureIssuance_b670956e-df4a-4733-963f-a696fc6e8714" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock_6b2784fa-6358-4a74-87bd-b0720078d261" xlink:href="kltr-20231231.xsd#kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_64767a94-1f4a-4612-9478-ddded354dc7d" xlink:to="loc_kltr_StockIssuedDuringPeriodSharesConversionOfWarrantsToCommonStock_6b2784fa-6358-4a74-87bd-b0720078d261" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails4EquityIncentivePlans" xlink:type="simple" xlink:href="kltr-20231231.xsd#STOCKHOLDERSEQUITYDetails4EquityIncentivePlans"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails4EquityIncentivePlans" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_aaa2f1ac-7d37-48a3-9e39-207a8cfaa0a5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_1582a2a7-bcc0-4b07-a22f-6a1cba8d6f22" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_aaa2f1ac-7d37-48a3-9e39-207a8cfaa0a5" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_1582a2a7-bcc0-4b07-a22f-6a1cba8d6f22" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementClassOfStockAxis_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementClassOfStockAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_1582a2a7-bcc0-4b07-a22f-6a1cba8d6f22" xlink:to="loc_us-gaap_StatementClassOfStockAxis_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementClassOfStockAxis_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e" xlink:to="loc_us-gaap_ClassOfStockDomain_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockDomain_9753e9d4-e244-40ce-b030-bd55b1148e07" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementClassOfStockAxis_20a8fcb4-9e68-4141-99d8-eb1a8a19cd4e" xlink:to="loc_us-gaap_ClassOfStockDomain_9753e9d4-e244-40ce-b030-bd55b1148e07" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameAxis_d008cfe8-03bb-46e4-b749-4b2f7d22b465" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_1582a2a7-bcc0-4b07-a22f-6a1cba8d6f22" xlink:to="loc_us-gaap_PlanNameAxis_d008cfe8-03bb-46e4-b749-4b2f7d22b465" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_d008cfe8-03bb-46e4-b749-4b2f7d22b465_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_PlanNameAxis_d008cfe8-03bb-46e4-b749-4b2f7d22b465" xlink:to="loc_us-gaap_PlanNameDomain_d008cfe8-03bb-46e4-b749-4b2f7d22b465_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PlanNameDomain_51c674a9-de3e-4cd1-80e4-7e980dc9a70b" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_PlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_PlanNameAxis_d008cfe8-03bb-46e4-b749-4b2f7d22b465" xlink:to="loc_us-gaap_PlanNameDomain_51c674a9-de3e-4cd1-80e4-7e980dc9a70b" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_A2020PlanMember_47dcf572-eb49-4499-9363-6c4cea7086c7" xlink:href="kltr-20231231.xsd#kltr_A2020PlanMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_PlanNameDomain_51c674a9-de3e-4cd1-80e4-7e980dc9a70b" xlink:to="loc_kltr_A2020PlanMember_47dcf572-eb49-4499-9363-6c4cea7086c7" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1a481f78-6395-4b7b-9349-e00b731695b0" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_aaa2f1ac-7d37-48a3-9e39-207a8cfaa0a5" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardOptionsGrantsInPeriodWeightedAverageExercisePrice_1a481f78-6395-4b7b-9349-e00b731695b0" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_kltr_ShareBasedPaymentArrangementPlanIncreaseOfAuthorizedSharesAsAPercentageOfOutstandingShares_94428d57-d452-405f-b774-06a3c5852144" xlink:href="kltr-20231231.xsd#kltr_ShareBasedPaymentArrangementPlanIncreaseOfAuthorizedSharesAsAPercentageOfOutstandingShares"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_aaa2f1ac-7d37-48a3-9e39-207a8cfaa0a5" xlink:to="loc_kltr_ShareBasedPaymentArrangementPlanIncreaseOfAuthorizedSharesAsAPercentageOfOutstandingShares_94428d57-d452-405f-b774-06a3c5852144" xlink:type="arc" order="1"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails8RSUs" xlink:type="simple" xlink:href="kltr-20231231.xsd#STOCKHOLDERSEQUITYDetails8RSUs"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails8RSUs" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ClassOfStockLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ScheduleOfStockByClassTable_440cd536-21a7-44bc-ab15-6e65fdff8e00" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ScheduleOfStockByClassTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ScheduleOfStockByClassTable_440cd536-21a7-44bc-ab15-6e65fdff8e00" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AwardTypeAxis_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AwardTypeAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_ScheduleOfStockByClassTable_440cd536-21a7-44bc-ab15-6e65fdff8e00" xlink:to="loc_us-gaap_AwardTypeAxis_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_AwardTypeAxis_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3949993a-1b28-4a52-81f2-33573bdd1ff8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_AwardTypeAxis_37ee9bf5-f7ff-4d5c-9e8e-73f95c4b24be" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3949993a-1b28-4a52-81f2-33573bdd1ff8" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestrictedStockMember_1cee28b1-a6dd-44a1-8102-4f5c743a3079" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestrictedStockMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ShareBasedCompensationArrangementsByShareBasedPaymentAwardAwardTypeAndPlanNameDomain_3949993a-1b28-4a52-81f2-33573bdd1ff8" xlink:to="loc_us-gaap_RestrictedStockMember_1cee28b1-a6dd-44a1-8102-4f5c743a3079" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_f9827cae-16b8-4601-9edc-5ef0e2cf6401" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedNumber_f9827cae-16b8-4601-9edc-5ef0e2cf6401" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_44ee58a8-20da-40f7-8957-d9a5be68c667" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriod_44ee58a8-20da-40f7-8957-d9a5be68c667" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_23bffabe-b735-4c04-a2d4-ed9c6b5bf480" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriod_23bffabe-b735-4c04-a2d4-ed9c6b5bf480" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_0dadc4ab-a6f6-4561-97bf-29f39af73ab3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeitedInPeriod_0dadc4ab-a6f6-4561-97bf-29f39af73ab3" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_94a74391-8fc2-41dc-bd86-e6a3599ce0f3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsGrantsInPeriodWeightedAverageGrantDateFairValue_94a74391-8fc2-41dc-bd86-e6a3599ce0f3" xlink:type="arc" order="4"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_5a05c132-c0df-4ceb-a318-4c474a32e4b2" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsVestedInPeriodWeightedAverageGrantDateFairValue_5a05c132-c0df-4ceb-a318-4c474a32e4b2" xlink:type="arc" order="5"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_11c8da65-741d-4574-9731-c3f925885b8f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsForfeituresWeightedAverageGrantDateFairValue_11c8da65-741d-4574-9731-c3f925885b8f" xlink:type="arc" order="6"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_d90b8b1e-80bc-45ff-9bd3-aedd03d6c5fe" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_ClassOfStockLineItems_8b651e44-b2c7-4592-9297-adf0f5d34b84" xlink:to="loc_us-gaap_ShareBasedCompensationArrangementByShareBasedPaymentAwardEquityInstrumentsOtherThanOptionsNonvestedWeightedAverageGrantDateFairValue_d90b8b1e-80bc-45ff-9bd3-aedd03d6c5fe" xlink:type="arc" order="7"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails9SBCExpense" xlink:type="simple" xlink:href="kltr-20231231.xsd#STOCKHOLDERSEQUITYDetails9SBCExpense"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/STOCKHOLDERSEQUITYDetails9SBCExpense" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_846077fc-6113-4c2f-ae3c-1856a38d5528" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_3f1db10b-e107-4697-902a-7405d5344f6f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_846077fc-6113-4c2f-ae3c-1856a38d5528" xlink:to="loc_us-gaap_StatementTable_3f1db10b-e107-4697-902a-7405d5344f6f" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationAxis_83a90732-dc05-41c2-a8b5-d3e2b0bb908f" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_3f1db10b-e107-4697-902a-7405d5344f6f" xlink:to="loc_us-gaap_IncomeStatementLocationAxis_83a90732-dc05-41c2-a8b5-d3e2b0bb908f" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_83a90732-dc05-41c2-a8b5-d3e2b0bb908f_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_83a90732-dc05-41c2-a8b5-d3e2b0bb908f" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_83a90732-dc05-41c2-a8b5-d3e2b0bb908f_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementLocationDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_IncomeStatementLocationAxis_83a90732-dc05-41c2-a8b5-d3e2b0bb908f" xlink:to="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfSalesMember_1ffb68d5-e775-418f-b443-a7d1ae81dc58" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_CostOfSalesMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:to="loc_us-gaap_CostOfSalesMember_1ffb68d5-e775-418f-b443-a7d1ae81dc58" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpenseMember_bcbcf8ce-1284-4e30-a72c-f3025ebae29e" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpenseMember_bcbcf8ce-1284-4e30-a72c-f3025ebae29e" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingAndMarketingExpenseMember_5b304cda-181d-4b52-a371-61627ffa71f3" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_SellingAndMarketingExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:to="loc_us-gaap_SellingAndMarketingExpenseMember_5b304cda-181d-4b52-a371-61627ffa71f3" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GeneralAndAdministrativeExpenseMember_1c143b2a-6f6e-42a8-9b4c-357d08404f9a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_GeneralAndAdministrativeExpenseMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementLocationDomain_c13a559a-cabe-4229-99c3-7f5aebaad5ee" xlink:to="loc_us-gaap_GeneralAndAdministrativeExpenseMember_1c143b2a-6f6e-42a8-9b4c-357d08404f9a" xlink:type="arc" order="3"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllocatedShareBasedCompensationExpense_d1dad101-02af-4db6-9e20-af49d9df20da" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AllocatedShareBasedCompensationExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_846077fc-6113-4c2f-ae3c-1856a38d5528" xlink:to="loc_us-gaap_AllocatedShareBasedCompensationExpense_d1dad101-02af-4db6-9e20-af49d9df20da" xlink:type="arc" order="0"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://www.corp.kaltura.com/role/ComprehensiveTextBlockListDetails_1" xlink:type="simple" xlink:href="kltr-20231231.xsd#ComprehensiveTextBlockListDetails_1"/>
  <link:definitionLink xlink:role="http://www.corp.kaltura.com/role/ComprehensiveTextBlockListDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementLineItems"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementTable_318ce0e6-3ddb-46ae-b9f2-31de9df8e777" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementTable"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:to="loc_us-gaap_StatementTable_318ce0e6-3ddb-46ae-b9f2-31de9df8e777" xlink:type="arc" order="1" xbrldt:closed="true" xbrldt:contextElement="segment"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StatementEquityComponentsAxis_64655f43-acfd-4e51-9236-fec2ac5923a6" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_StatementEquityComponentsAxis"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/hypercube-dimension" xlink:from="loc_us-gaap_StatementTable_318ce0e6-3ddb-46ae-b9f2-31de9df8e777" xlink:to="loc_us-gaap_StatementEquityComponentsAxis_64655f43-acfd-4e51-9236-fec2ac5923a6" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_64655f43-acfd-4e51-9236-fec2ac5923a6_default" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-default" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_64655f43-acfd-4e51-9236-fec2ac5923a6" xlink:to="loc_us-gaap_EquityComponentDomain_64655f43-acfd-4e51-9236-fec2ac5923a6_default" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EquityComponentDomain_d12962ed-6079-4aa1-9322-b77089050155" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_EquityComponentDomain"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/dimension-domain" xlink:from="loc_us-gaap_StatementEquityComponentsAxis_64655f43-acfd-4e51-9236-fec2ac5923a6" xlink:to="loc_us-gaap_EquityComponentDomain_d12962ed-6079-4aa1-9322-b77089050155" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_d5cb333a-938f-4c91-b80e-20a6996c4709" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d12962ed-6079-4aa1-9322-b77089050155" xlink:to="loc_us-gaap_AccumulatedNetUnrealizedInvestmentGainLossMember_d5cb333a-938f-4c91-b80e-20a6996c4709" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_ebac62d6-0b10-4d22-bd05-dc62476871f5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_EquityComponentDomain_d12962ed-6079-4aa1-9322-b77089050155" xlink:to="loc_us-gaap_AccumulatedGainLossNetCashFlowHedgeParentMember_ebac62d6-0b10-4d22-bd05-dc62476871f5" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_9540c794-00a7-4b52-9209-7138bc562ce5" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:to="loc_us-gaap_AccumulatedOtherComprehensiveIncomeLossNetOfTax_9540c794-00a7-4b52-9209-7138bc562ce5" xlink:type="arc" order="0"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_dfcc4c78-4c94-43a3-8121-bc89485d5c9a" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossBeforeReclassificationsNetOfTax_dfcc4c78-4c94-43a3-8121-bc89485d5c9a" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_39557574-1834-4c97-98e6-4d4040df1a17" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:to="loc_us-gaap_ReclassificationFromAccumulatedOtherComprehensiveIncomeCurrentPeriodNetOfTax_39557574-1834-4c97-98e6-4d4040df1a17" xlink:type="arc" order="2"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_f1a4e1f1-0c9c-42de-b3fc-e5d4762a1dd8" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_OtherComprehensiveIncomeLossNetOfTax"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_StatementLineItems_a4cff070-835f-4bfa-b093-4d28b88257c8" xlink:to="loc_us-gaap_OtherComprehensiveIncomeLossNetOfTax_f1a4e1f1-0c9c-42de-b3fc-e5d4762a1dd8" xlink:type="arc" order="3"/>
  </link:definitionLink>
  <link:roleRef roleURI="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="simple" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-roles-2023.xsd#eedm"/>
  <link:definitionLink xlink:role="http://fasb.org/us-gaap/role/eedm/ExtensibleEnumerationLists" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeStatementAbstract" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_IncomeStatementAbstract"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementAbstract" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense" xlink:type="arc" order="1"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RestructuringCosts" xlink:href="https://xbrl.fasb.org/us-gaap/2023/elts/us-gaap-2023.xsd#us-gaap_RestructuringCosts"/>
    <link:definitionArc xlink:arcrole="http://xbrl.org/int/dim/arcrole/domain-member" xlink:from="loc_us-gaap_IncomeStatementAbstract" xlink:to="loc_us-gaap_RestructuringCosts" xlink:type="arc" order="2"/>
  </link:definitionLink>
</link:linkbase>
